| 基于高通量测序的不同生态环境东方泽泻块茎转录组分析 |
| 投稿时间：2022-04-27 点此下载全文
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| 引用本文：韦陈彬,谷巍,田荣,邱蓉丽,韩赟,李桃,季媛媛,朱瑜.基于高通量测序的不同生态环境东方泽泻块茎转录组分析[J].中国现代中药,2022,24(12):2342-2349 |
| DOI：10.13313/j.issn.1673-4890.20220427002 |
| 摘要点击次数: 150 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 韦陈彬 |
WEI Chen-bin |
南京中医药大学 药学院，江苏 南京 210023 |
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| 谷巍* |
GU Wei |
南京中医药大学 药学院，江苏 南京 210023
南京中医药大学 江苏省中药资源产业化过程协同创新中心，江苏 南京 210023 |
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| 田荣 |
TIAN Rong |
南京中医药大学 药学院，江苏 南京 210023 |
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| 邱蓉丽 |
QIU Rong-li |
南京中医药大学 药学院，江苏 南京 210023 |
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| 韩赟 |
HAN Yun |
南京中医药大学 附属苏州市中医医院，江苏 苏州 215009 |
Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou 215009, China |
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| 李桃 |
LI Tao |
南京中医药大学 药学院，江苏 南京 210023 |
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| 季媛媛 |
JI Yuan-yuan |
南京中医药大学 药学院，江苏 南京 210023 |
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| 朱瑜 |
ZHU Yu |
南京中医药大学 药学院，江苏 南京 210023 |
School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| 基金项目:国家自然科学基金项目（82073958，81673534） |
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| 中文摘要:目的 探索不同生态环境对东方泽泻生理活动及其药效成分原萜烷型三萜生物合成调控的分子基础。方法 通过Illumina HiSeq测序平台获取种植于福建和四川的东方泽泻块茎转录组数据并进行功能注释，基于|log2FC|>0.58（FC为差异倍数）、P<0.05的筛选条件获得差异表达基因。结果 转录组测序共获得50 652条unigenes，有22 689条unigenes注释到京都基因与基因组百科全书（KEGG）、NR、SwissProt、COG、基因本体（GO）和Pfam数据库。从福建和四川2种环境生长的东方泽泻之间筛选到3878条差异表达基因，其中2082条在福建东方泽泻中上调表达，1796条在四川东方泽泻中上调表达，差异基因主要参与植物防御反应、光合作用、淀粉和蔗糖代谢、萜类生物合成等生理活动，包括5个原萜烷型三萜生物合成通路上的关键酶基因。此外，东方泽泻移栽四川后，与三萜生物合成相关的转录因子和细胞色素P450（CYP450）基因表达也发生了显著变化。结论 利用高通量测序技术和生物信息分析获得了不同生态环境下东方泽泻转录组信息特征，为阐明生态环境对东方泽泻原萜烷型三萜生物合成的调控机制提供参考。 |
| 中文关键词:东方泽泻 生态环境 转录组学 原萜烷型三萜 |
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| Transcriptome Data Analysis of Alisma orientale Tubers Grown in Different Ecological Environments by High-Throughput Sequencing |
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| Abstract:Objective To explore the molecular mechanism of different ecological environments on the regulation of physiological activities and biosynthesis of protostane triterpenes of Alisma orientale.Methods The transcriptome data of A. orientale tubers from Fujian and Sichuan were obtained by Illumina HiSeq sequencing platform, followed by functional annotation, and differentially expressed genes (DEGs) were obtained based on the screening conditions of |log2FC|>0.58 and P<0.05.Results A total of 50 652 unigenes were obtained by transcriptome sequencing, and 22 689 unigenes were annotated to Kyoto Encyclopedia of Genes and Genomes (KEGG), NR, SwissProt, COG, Gene Ontology (GO), and Pfam. A total of 3878 DEGs were screened out from A. orientale grown in Fujian and Sichuan, including 2082 with up-regulated expression in Fujian and 1796 with up-regulated expression in Sichuan. DEGs were mainly involved in physiological activities such as plant defense response, photosynthesis, starch and sucrose metabolism, and terpene biosynthesis, including five key enzyme genes involved in the biosynthesis of protostane triterpenes. In addition, the expression of transcription factors and CYP450 genes related to triterpene biosynthesis also changed significantly after A. orientale transplantation to Sichuan.Conclusion The high-throughput sequencing technology and bioinformatics analysis were used to obtain the transcriptome information characteristics of A. orientale under different ecological environments, which laid a foundation for clarifying the regulation mechanism of ecological environment on the biosynthesis of protostane triterpenes of A. orientale. |
| keywords:Alisma orientale (Sam.) Juzep. ecological environment transcriptomics protostane triterpene |
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