| UPLC-MS/MS同时检测人参健脾丸和人参归脾丸中非法添加西洋参 |
| 投稿时间：2022-01-27 点此下载全文
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| 引用本文：姚宝林,王然然,张淼,韦晶晶,尹贻珍,韩慧琴.UPLC-MS/MS同时检测人参健脾丸和人参归脾丸中非法添加西洋参[J].中国现代中药,2023,25(1):150-156 |
| DOI：10.13313/j.issn.1673-4890.20220127004 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 姚宝林 |
YAO Bao-lin |
承德市食品药品检验检测中心，河北 承德 067000 |
Chengde City Institute for Food and Drug Control, Chengde 067000, China |
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| 王然然 |
WANG Ran-ran |
承德市食品药品检验检测中心，河北 承德 067000 |
Chengde City Institute for Food and Drug Control, Chengde 067000, China |
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| 张淼 |
ZHANG Miao |
石家庄市食品药品检验中心，河北 石家庄 050011 |
Shijiazhuang Institute for Food and Drug Control, Shijiazhuang 050011, China |
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| 韦晶晶 |
WEI Jing-jing |
张家口市食品药品检验中心，河北 张家口 075000 |
Zhangjiakou City Institute for Food and Drug Control, Zhangjiakou 075000, China |
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| 尹贻珍 |
YIN Yi-zhen |
张家口市食品药品检验中心，河北 张家口 075000 |
Zhangjiakou City Institute for Food and Drug Control, Zhangjiakou 075000, China |
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| 韩慧琴* |
HAN Hui-qin |
张家口市食品药品检验中心，河北 张家口 075000 |
Zhangjiakou City Institute for Food and Drug Control, Zhangjiakou 075000, China |
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| 基金项目:张家口市重点研发计划项目（2121037D） |
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| 中文摘要:目的 建立一种可同时测定人参归脾丸和人参健脾丸中非法添加西洋参的超高效液相色谱串联质谱法（UPLC-MS/MS）检测方法。方法 用80%甲醇回流提取制备供试品溶液，使用Waters ACQUITY UPLC HSS T3 C18色谱柱（100 mm×2.1 mm，1.8 μm），乙腈-水为流动相进行梯度洗脱，流速为0.35 mL·min–1，柱温为40 ℃，采用电喷雾离子源、多反应监测模式、负离子方式进行扫描。结果 拟人参皂苷F11在5.025~100.500 ng·mL–1线性关系良好（r=0.998 5），重复性RSD为1.4%，拟人参皂苷F11在人参归脾丸和人参健脾丸中的回收率分别为97.71%、97.64%，检出限为1 ng·mL–1，定量限为3 ng·mL–1。结论 建立的方法灵敏度高、实用性强，可作为人参归脾丸和人参健脾丸中非法投入西洋参的补充检验方法。 |
| 中文关键词:超高效液相色谱串联质谱法 人参健脾丸 人参归脾丸 西洋参 拟人参皂苷F11 |
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| Simultaneous Determination of Panax quinquefolium Illegally Added in Renshen Jianpi Pills and Renshen Guipi Pills by UPLC-MS/MS |
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| Abstract:Objective To establish a UPLC-MS/MS method for simultaneous determination of Panax quinquefolium illegally added in Renshen Jianpi Pills and Renshen Guipi Pills.Methods The chromatographic separation was performed on Waters ACQUITY UPLC HSS T3 C18 (100 mm×2.1 mm, 1.8 μm) with acetonitrile and water as the mobile phases for gradient elution at a flow rate of 0.35 mL·min–1. The column temperature was 40 ℃, and the scanning was performed using electrospray ionization (ESI) source in multiple reaction monitoring (MRM) mode and negative ion mode.Results Pseudo-ginsenoside F11 showed a good linearity in the concentration range of 5.025-100.500 ng·mL–1 (r=0.998 5), and the relative standard deviation (RSD) of the repeatability test was 1.4%. The average recovery of pseudo-ginsenoside F11 was 97.71% in Renshen Guipi Pills and 97.64% in Renshen Jianpi Pills. The detection limit and the quantification limit was 1 ng·mL–1and 3 ng·mL–1, respectively.Conclusion The established method was highly sensitive and practical, and could be used as a supplementary test method for the illegal input of P. quinquefolium in Renshen Guipi Pills and Renshen Jianpi Pills. |
| keywords:UPLC-MS/MS Renshen Jianpi Pills Renshen Guipi Pills Panax quinquefolium pseudo-ginsenoside F11 |
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