肝肾细胞毒性导引下鱼腥草中内酰胺生物碱的分离和鉴定
投稿时间:2022-06-23     点此下载全文
引用本文:武营雪,康帅,李妍,刘静,戴忠,马双成.肝肾细胞毒性导引下鱼腥草中内酰胺生物碱的分离和鉴定[J].中国现代中药,2023,25(3):544-548
DOI:10.13313/j.issn.1673-4890.20220623007
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作者中文名作者英文名单位中文名单位英文名E-Mail
武营雪 WU Ying-xue 中国食品药品检定研究院,北京 100050 National Institutes for Food and Drug Control, Beijing 100050, China  
康帅 KANG Shuai 中国食品药品检定研究院,北京 100050 National Institutes for Food and Drug Control, Beijing 100050, China  
李妍 LI Yan 中国医学科学院 医药生物技术研究所,北京 100050 Institute of Medical Biotechnology, Chinese Academy of Medical Sciences, Beijing 100050, China  
刘静* LIU Jing 中国食品药品检定研究院,北京 100050 National Institutes for Food and Drug Control, Beijing 100050, China  
戴忠 DAI Zhong 中国食品药品检定研究院,北京 100050 National Institutes for Food and Drug Control, Beijing 100050, China  
马双成 MA Shuang-cheng 中国食品药品检定研究院,北京 100050 National Institutes for Food and Drug Control, Beijing 100050, China  
基金项目:国家药典委员会课题(2021Z15)
中文摘要:目的 对鱼腥草Houttuynia cordata Thunb.地上部分化学成分及其肝、肾细胞毒性进行研究。方法 采用CCK-8法测定鱼腥草不同极性段萃取物对6种肝、肾细胞(小鼠正常肝细胞AML-12、大鼠肝细胞BRL-3A、人正常肝细胞L-02、大鼠肾小管上皮细胞NRK-52E、非洲绿猴肾细胞Vero和人肾皮质近曲小管上皮细胞HK-2)的毒性。利用硅胶、中性氧化铝、MCI和制备高效液相色谱法(HPLC)分离纯化,结合质谱(MS)和核磁共振(NMR)鉴定化合物的结构。结果 鱼腥草二氯甲烷部位较其他部位具有明显细胞毒性;从中分离并鉴定了6个化合物,分别为3,5-二癸酰基-4-壬基-1,4-二氢吡啶(1)、马兜铃内酰胺AⅡ(2)、马兜铃内酰胺FⅠ(3)、顺式-N-(4-羟基苯乙烯基)苯酰胺(4)、马兜铃内酰胺BⅡ(5)、头花千金藤二酮B(6)。鱼腥草二氯甲烷部位对AML-12、BRL-3A、L-02和NRK-52E细胞具有明显的细胞毒性;化合物235对部分细胞株具有一定的细胞毒性,化合物6对6种测试细胞均具有一定细胞毒性作用。结论 鱼腥草中内酰胺生物碱具有一定肝、肾细胞毒性。
中文关键词:鱼腥草  马兜铃内酰胺  生物碱  化学成分  安全性
 
Isolation and Identification of Lactams from Houttuynia cordata Thunb. under Guidance of Hepatorenal Cytotoxicity
Abstract:Objective To study the chemical components from the aerial part of Houttuynia cordata Thunb. and its hepatorenal cytotoxicity.Methods Different polar extracts were evaluated for their hepatorenal cytotoxicity in normal murine hepatocytes AML-12, rat hepatocytes BRL-3A, normal human hepatocytes L-02, rat renal tubular epithelial cells NRK-52E, African green monkey kidney cells Vero and human renal cortex proximal tubule epithelial cells HK-2 cell lines by cell counting kit-8 (CCK-8) assay. The chemical constituents were separated and identified by column chromatography on silica gel, neutral aluminum oxide, middle chromatogram isolated gel (MCI) and preparative high-performance liquid chromatograph (HPLC), while their structures were determined by physicochemical properties and spectroscopic data.Results The dichloromethane fraction exhibited higher cytotoxicity as compared with other fractions. Six compounds were isolated from the dichloromethane fraction of the aerial parts of H. cordata Thunb., and identified as 3,5-didecanoyl-4-nonyl-1,4-dihydropyridine (1), aristololactam AⅡ (2), aristololactam AⅡ (3), cis-N-(4-hydroxystyryl)benzamide (4), aristololactam BⅡ (5), cepharadione B (6). The dichloromethane fraction exhibited high cytotoxicity against AML-12, BRL-3A, L-02 and NRK-52E cells. Compounds 2, 3, and 5 showed certain cytotoxicity to some cells. Compound 6 showed certain cytotoxicity to all the six cell lines.Conclusion The lactams from H. cordata Thunb. had certain hepatorenal cytotoxicity.
keywords:Houttuynia cordata Thunb.  aristololactams  alkaloids  chemical components  safety
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