| 奇楠沉香超临界提取物的化学成分研究 |
| 投稿时间：2023-03-24 点此下载全文
|
| 引用本文：陈德力,马国需,刘会梅,王灿红,刘洋洋,杨云,魏建和.奇楠沉香超临界提取物的化学成分研究[J].中国现代中药,2023,25(9):1925-1933 |
| DOI：10.13313/j.issn.1673-4890.20230324002 |
| 摘要点击次数: 70 |
| 全文下载次数: 0 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 陈德力 |
CHEN De-li |
中国医学科学院 北京协和医学院 药用植物研究所 海南分所/海南省南药资源保护与开发重点实验室， 海南 海口 570311 |
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China |
|
| 马国需 |
MA Guo-xu |
中国医学科学院 北京协和医学院 药用植物研究所 海南分所/海南省南药资源保护与开发重点实验室， 海南 海口 570311
中国医学科学院 北京协和医学院 药用植物研究所，北京 100193 |
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China |
|
| 刘会梅 |
LIU Hui-mei |
中国医学科学院 北京协和医学院 药用植物研究所 海南分所/海南省南药资源保护与开发重点实验室， 海南 海口 570311 |
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China |
|
| 王灿红 |
WANG Can-hong |
中国医学科学院 北京协和医学院 药用植物研究所 海南分所/海南省南药资源保护与开发重点实验室， 海南 海口 570311 |
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China |
|
| 刘洋洋 |
LIU Yang-yang |
中国医学科学院 北京协和医学院 药用植物研究所 海南分所/海南省南药资源保护与开发重点实验室， 海南 海口 570311 |
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China |
|
| 杨云* |
YANG Yun |
中国医学科学院 北京协和医学院 药用植物研究所 海南分所/海南省南药资源保护与开发重点实验室， 海南 海口 570311 |
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China |
|
| 魏建和 |
WEI Jian-he |
中国医学科学院 北京协和医学院 药用植物研究所 海南分所/海南省南药资源保护与开发重点实验室， 海南 海口 570311
中国医学科学院 北京协和医学院 药用植物研究所，北京 100193 |
Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China |
|
|
| 基金项目:海南省重点研发计划项目（ZDYF2020111，ZDYF2022SHFZ030）；国家自然科学基金项目（82204657）；中国医学科学院医学与健康科技创新工程项目（2021-I2M-1-032） |
|
| 中文摘要:目的 研究奇楠沉香超临界提取物的化学成分。方法 采用硅胶柱色谱、薄层色谱及半制备液相色谱等技术对奇楠沉香超临界提取物进行分离纯化，结合谱学手段和文献数据鉴定化合物的结构。体外测定化合物对皮质酮（CORT）诱导的PC12细胞损伤的保护作用。结果 从奇楠沉香超临界提取物中分离得到17个化合物，分别鉴定为12,15-二酮基-α-芹子烯（1）、15-酮基-桉叶烷-4,11(13)-二烯-12-甲酯（2）、petafolia A（3）、12-羟基-4(5),11(13)-桉叶二烯-15-醛（4）、12,15-二酮基-芹子-4,11-二烯（5）、(7S,8R,10S)-(+)-8,12-二羟基-芹子-4,11-二烯-14-醛（6）、白木香酸（7）、1α-羟基-7βH-荒漠木-9,11-二烯-8-酮（8）、7α-H-9(10)-烯-11,12-环氧-8-氧代荒漠木烷（9）、petafolia B（10）、11-羟基-瓦伦-1(10)-烯-2-酮（11）、白木香醇（12）、2-(2-苯乙基)色酮（13）、奇楠沉香酮A（14）、4′-甲氧基-2-(2-苯乙基)色酮（15）、6-甲氧基-2-[2-(4′-甲氧基苯乙基)色酮（16）和丁香醛（17）。CORT诱导细胞损伤后的细胞存活率为48.78%，给予受试药物后，不同化合物不同质量浓度下细胞存活率出现不同程度的升高，存活率为50.35%~91.36%。结论 化合物1~17均为首次从奇楠沉香超临界提取物中分离得到，化合物2和4对CORT诱导的PC12细胞损伤的保护活性较强。 |
| 中文关键词:奇楠沉香 超临界 倍半萜 色酮 神经保护 |
| |
| Chemical Constituents of "Qi-Nan" Agarwood from Aquilaria sinensis |
|
|
| Abstract:Objective To study the chemical constituents of "Qi-Nan" agarwood from Aquilaria sinensis.Methods The compounds were isolated and purified by silica gel column chromatography, thin layer chromatography, and semi-preparative high performance liquid chromatography, and their chemical structures were identified by spectral techniques and comparison with literature data. Furthermore, the protective effects of the compounds on the PC12 cells exposed to corticosterone (CORT) were determined.Results Seventeen compounds were isolated from "Qi-Nan" agarwood and identified as 12,15-dioxo-α-selinen (1), methyl-15-oxo-eudesmane-4,11(13)-dien-12-oate (2), petafolia A (3), 12-hydroxy-4(5),11(13)-eudesmadien-15-al (4), 12,15-dioxo-selina-4,11-dine (5), (7S,8R,10S)-(+)-8,12-dihydroxy-selina-4,11-dien-14-al (6), baimuxifuranic acid (7), 1α-hydroxy-7βH-eremophil-9,11-dien-8-one (8), 7α-H-9(10)-ene-11,12-epoxy-8-oxoeremophilane (9), petafolia B (10), 11-hydroxy-valenc-1(10)-en-2-one (11), baimuxinol (12), 2-(2-phenylethyl)chromone (13), qinanone A (14), 4′-methoxy-2-(2-phenylethyl)chromone (15), 6-methoxy-2-[2-(4′-methoxyphenyl)ethyl] chromone (16), and syringaldehyde (17). The survival of the cells exposed to CORT was 48.78%, and it increased to 50.35%-91.36% after treatment with different compounds at different concentrations.Conclusions Compounds 1-17 were isolated from the supercritical extract of "Qi-Nan" agarwood from A. sinensis for the first time, and compounds 2 and 4 showed strong neuroprotective activities against CORT-induced damage in PC12 cells. |
| keywords:"Qi-Nan" agarwood supercritical extraction sesquiterpenoid chromone neuroprotective |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
