| 一测多评法同时测定菊花配方颗粒中8个成分的含量 |
| 投稿时间：2023-01-31 点此下载全文
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| 引用本文：赵剑锋,宋宗华,刘静.一测多评法同时测定菊花配方颗粒中8个成分的含量[J].中国现代中药,2023,25(9):1979-1984 |
| DOI：10.13313/j.issn.1673-4890.20230131002 |
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| 中文摘要:目的 建立一测多评法（QAMS）同时测定菊花配方颗粒中绿原酸、1,3-O-二咖啡酰基奎宁酸、芦丁、木犀草苷、木犀草素-7-O-β-D-葡萄糖醛酸苷、3,4-O-二咖啡酰基奎宁酸、3,5-O-二咖啡酰基奎宁酸、4,5-O-二咖啡酰基奎宁酸的含量。方法 采用高效液相色谱法进行含量测定，色谱柱为Agilent TC-C18 （250 mm×4.6 mm，5 μm），以乙腈-0.1% 磷酸水溶液为流动相梯度洗脱，检测波长为348 nm，流速为1.0 mL·min–1，柱温为30 ℃，进样量为10 μL。以木犀草苷为内标物，采用多点校正法计算其他7个成分的相对校正因子，测定8个成分的含量，并比较与外标法测定结果的差异。结果 8个成分在一定质量浓度范围内与峰面积呈良好的线性关系，平均加样回收率为96.7%~102.1%，RSD为1.8%~2.9%。QAMS与外标法测定7批菊花配方颗粒中8个成分含量差异无统计学意义。结论 建立的QAMS简便快速，可以更全面地控制菊花配方颗粒的质量。 |
| 中文关键词:菊花 菊花配方颗粒 一测多评 相对校正因子 |
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| Simultaneous Determination of Eight Components in Juhua Formula Granules by QAMS |
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| Abstract:Objective To establish a quantitative analysis of multiple components with a single marker (QAMS) method for simultaneous determination of the content of chlorogenic acid, 1,3-O-dicaffeoylquinic, rutin, luteoloside, luteolin-7-O-β-D-glucuronide, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid in Juhua Formula Granules.Methods High-performance liquid chromatography (HPLC) was used for content determination. The chromatographic column was Agilent TC-C18 (250 mm×4.6 mm, 5 μm), and the mobile phase consisted of acetonitrile and 0.1% phosphoric acid aqueous solution in gradient elution. The detection wavelength was set at 348 nm, the flow rate was 1.0 mL·min–1, the column temperature was 30 °C, and the injection volume was 10 μL. Luteoloside was used as an internal standard, and the relative correction factors of the other seven components were calculated using a multi-point calibration method. The content of the eight components was determined, and the differences between the results obtained by QAMS and the external standard method were compared.Results The eight components showed good linearity within a certain range, with average recovery rates ranging from 96.7% to 102.1%, and RSDs ranging from 1.8% to 2.9%. There was no statistically significant difference in the content of the eight components between QAMS and the external standard method in seven batches of Chrysanthemum formula granules.Conclusion The established QAMS method is simple and rapid, and it can comprehensively control the quality of Juhua Formula Granules. |
| keywords:Chrysanthemum morifolium Ramat. Juhua formula granules QAMS RCF |
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