蜜环菌耐低温菌株的筛选及生理机制探讨
投稿时间:2023-04-27     点此下载全文
引用本文:黄萱,徐娇,李胜男,欧小宏,袁青松,江维克,王艳红,周涛.蜜环菌耐低温菌株的筛选及生理机制探讨[J].中国现代中药,2023,25(12):2560-2567
DOI:10.13313/j.issn.1673-4890.20230427005
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作者中文名作者英文名单位中文名单位英文名E-Mail
黄萱 HUANG Xuan 贵州农业职业学院 食品与药品系,贵州 贵阳 551400
贵州中医药大学 中药民族药资源研究院,贵州 贵阳 550025
Department of Food and Drug, Guizhou Agricultural Vocational College, Guiyang 551400, China
Resource Institute for Chinese & Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
 
徐娇 XU Jiao 贵州中医药大学 中药民族药资源研究院,贵州 贵阳 550025 Resource Institute for Chinese & Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China  
李胜男 LI Sheng-nan 贵州中医药大学 中药民族药资源研究院,贵州 贵阳 550025 Resource Institute for Chinese & Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China  
欧小宏 OU Xiao-hong 贵州中医药大学 中药民族药资源研究院,贵州 贵阳 550025 Resource Institute for Chinese & Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China  
袁青松 YUAN Qing-song 贵州中医药大学 中药民族药资源研究院,贵州 贵阳 550025
道地药材国家重点实验室,北京 100700
Resource Institute for Chinese & Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China
State Key Laboratory of Dao-di Herbs, Beijing 100700, China
 
江维克 JIANG Wei-ke 贵州中医药大学 中药民族药资源研究院,贵州 贵阳 550025 Resource Institute for Chinese & Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China  
王艳红 WANG Yan-hong 贵州中医药大学 中药民族药资源研究院,贵州 贵阳 550025 Resource Institute for Chinese & Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China  
周涛* ZHOU Tao 贵州中医药大学 中药民族药资源研究院,贵州 贵阳 550025 Resource Institute for Chinese & Ethnic Materia Medica, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China  
基金项目:国家自然科学地区基金项目(81960694,32060080);贵州省教育厅高校科研平台项目(黔教技〔2022〕021);贵州省自然科学基金项目(黔科合基础-ZK〔2021〕一般523);中央本级重大增减支项目(2060302)
中文摘要:目的 探究低温胁迫对不同蜜环菌生长的影响,为高海拔地区天麻种植选育耐低温的优良蜜环菌菌株提供科学依据。方法 以不同天麻产区的24株蜜环菌为实验材料,采用rDNA-基因间隔区(IGS)序列进行分析鉴定,确定供试菌株的亲缘关系;以23 ℃为对照(CK)、15 ℃为低温胁迫,利用赋值法综合评价各菌株生长速率、生物量、菌索长度,筛选耐低温的优良菌株,采用蒽酮硫酸法检测可溶性糖含量,利用SPSS 26.0分析低温胁迫可溶性糖含量与生长特性的相关性。结果 24株蜜环菌均与高卢蜜环菌Armillaria gallica亲缘关系较相近。低温胁迫后,各菌株的生物量、菌索长度、生长速率均受到不同程度的抑制,根据赋值法综合评价,有4株菌株的耐低温能力较强、可溶性糖含量均增加,而对于低温较为敏感的菌株可溶性糖含量均降低。相关性分析结果表明,可溶性糖含量与生物量、菌索长度、生长速率呈强正相关。结论 高卢蜜环菌为天麻生产中常用伴生菌种,YN4、GZ7、GZ17、SX2菌株的耐低温能力较强,推测蜜环菌通过累积可溶性糖抵御低温胁迫。
中文关键词:蜜环菌  天麻  低温胁迫  生长特性  可溶性糖含量
 
Screening and Physiological Mechanism Exploration of Low-temperature Resistant Strains of Armillaria
Abstract:Objective To explore the effects of low-temperature stress on the growth of different Armillaria strains and provide a scientific basis for the breeding of low-temperature resistant Armillaria strains for Gastrodia elata in high-altitude areas.Methods A total of 24 strains of Armillaria from different Gastrodia elata producing areas were used as experimental materials, and rDNA-IGS sequence analysis was used for identification to further determine the genetic relationship of the tested strains. The growth rate, biomass, and mycelial length were comprehensively evaluated by assignment method with 23 ℃ as the control condition and 15 ℃ as the stress condition, so as to screen excellent strains with low-temperature resistance. The soluble sugar content was detected by anthrone and sulfuric acid method, and the correlation between soluble sugar content and growth characteristics under low-temperature stress was analyzed by SPSS 26.0.Results All 24 strains of Armillaria had the closest relationship with Armillaria gallica. After low-temperature stress, the biomass, mycelial length, and growth rate of each strain were inhibited to different degrees. According to the comprehensive evaluation of the assignment method, there were four strains with strong resistance to low temperature, and the soluble sugar content of the strains increased, while the soluble sugar content of the strains that were more sensitive to low temperature decreased. Correlation analysis showed that soluble sugar content had a strong positive correlation with biomass, mycelial length, and growth rate.Conclusion Armillaria gallica was the main species used in the production of Gastrodia elata. YN4, GZ7, GZ17, and SX2 strains showed strong resistance to low temperature, suggesting that Armillaria could resist low-temperature stress by accumulating higher soluble sugar content.
keywords:Armillaria  Gastrodia elata Bl.  low-temperature stress  growth characteristics  soluble sugar content
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