基于多组分定量联合PCA、OPLS-DA及EW-TOPSIS分析的癣湿药水综合质量评价
投稿时间:2023-09-28     点此下载全文
引用本文:冉令超,卢立欣,冯金鹰,李志明,纪康康.基于多组分定量联合PCA、OPLS-DA及EW-TOPSIS分析的癣湿药水综合质量评价[J].中国现代中药,2024,26(4):726-732
DOI:10.13313/j.issn.1673-4890.20230928003
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作者中文名作者英文名单位中文名单位英文名E-Mail
冉令超 RAN Ling-chao 山东省立第三医院 药学部,山东 济南 250000 Department of Pharmacy, Shandong Provincial Third Hospital, Jinan 250000, China  
卢立欣* LU Li-xin 山东省立第三医院 药学部,山东 济南 250000 Department of Pharmacy, Shandong Provincial Third Hospital, Jinan 250000, China  
冯金鹰 FENG Jin-ying 山东省立第三医院 药学部,山东 济南 250000 Department of Pharmacy, Shandong Provincial Third Hospital, Jinan 250000, China  
李志明 LI Zhi-ming 山东中医药大学 药学院,山东 济南 250355 School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China  
纪康康 JI Kang-kang 山东省立第三医院 药学部,山东 济南 250000 Department of Pharmacy, Shandong Provincial Third Hospital, Jinan 250000, China  
基金项目:山东省中医药科技发展计划项目(2019-0330)
中文摘要:目的 建立癣湿药水多组分定量检测方法,通过主成分分析法(PCA)、正交偏最小二乘法-判别分析(OPLS-DA)联合熵权优劣解距离法(EW-TOPSIS)对多组分含量测定结果进行差异性分析,评价其综合质量。方法 采用高效液相色谱法,Global Chromatography C18色谱柱,以乙腈-0.1%磷酸水溶液为流动相,同时测定12批癣湿药水中柠檬苦素、吴茱萸碱、吴茱萸次碱、欧前胡素、蛇床子素、异欧前胡素、升麻素、5-O-甲基维斯阿米醇苷、土荆皮丙酸、土荆皮乙酸、羟基-α-山椒素、羟基-β-山椒素、羟基-γ-山椒素含量,通过化学计量学和EW-TOPSIS对定量结果进行分析。结果 13个成分在各自范围内线性关系良好(r>0.999),平均加样回收率为96.90%~100.09%(n=9,RSD<2.0%)。PCA结果显示12批癣湿药水聚为3类;OPLS-DA结果表明蛇床子素、土荆皮乙酸、羟基-α-山椒素、升麻素、柠檬苦素和欧前胡素为癣湿药水差异特征标志物;EW-TOPSIS结果显示欧氏贴近度为0.188 7~0.692 0,不同企业间产品13个成分含量存在一定差异。结论 所建立方法操作便捷、结果准确,可用于癣湿药水质量的综合评价,多组分定量联合PCA、OPLS-DA及EW-TOPSIS亦可用于其他中药制剂检测及质量评价中。
中文关键词:癣湿药水  高效液相色谱法  主成分分析  正交偏最小二乘法-判别分析  熵权优劣解距离法
 
Comprehensive Quality Evaluation of Xianshi Lotion Based on Multi-component Quantitative Combined with PCA, OPLS-DA and EW-TOPSIS Analysis
Abstract:Objective To establish a multi-component quantitative detection method of Xianshi Lotion, principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), combined with entropy weight-technique for order preference by similarity to ideal solution (EW-TOPSIS) method was applied to analyze the differences in the results of multi-component content and evaluate its comprehensive quality.Methods HPLC was used with Global Chromatography C18 column as the chromatographic column and acetonitrile-0.1% phosphoric acid as the mobile phase. The contents of limonin, evodiamine, rutaecarpine, imperatorin, osthole, isoimperatorin, cimifugin, 5-O-methylvisammioside, pseudolaric acid C, pseudolaric acid B, hydroxy-α-sanshool, hydroxy-β-sanshool and hydroxy-γ- sanshool in 12 batches of Xianshi Lotion were determined. The quantitative results were analyzed by chemometrics and EW-TOPSIS.Results The 13 components had good linear relationships within their respective ranges (r>0.999), and the average recovery was 96.90%-100.09% (n=9, RSD<2.0%). The results of PCA showed that 12 batches of Xianshi Lotion were divided into 3 groups. The results of OPLS-DA showed that osthole, pseudolaric acid B, hydroxy-α-sanshool, cimifugin, limonin and imperatorin were the differential characteristic markers of Xianshi Lotion. The EW-TOPSIS analysis results showed that the Euclid approach degree Ci value was between 0.188 7-0.692 0. There were some differences in the content of 13 components in products between different enterprises.Conclusion The established method is convenient and accurate, and can be used for the comprehensive evaluation of the quality of Xianshi Lotion. The multi-component quantitative combined with PCA, OPLS-DA and EW-TOPSIS methods can also be used for the detection and quality evaluation of other traditional Chinese medicine preparations.
keywords:Xianshi Lotion  HPLC  PCA  OPLS-DA  EW-TOPSIS
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