MTT法用于中药成分细胞毒性和增殖检测的干扰因素及控制措施
投稿时间:2023-12-12     点此下载全文
引用本文:褚皓天,赵鹏,田燕歌,任周新.MTT法用于中药成分细胞毒性和增殖检测的干扰因素及控制措施[J].中国现代中药,2024,26(4):749-756
DOI:10.13313/j.issn.1673-4890.20231212001
摘要点击次数: 124
全文下载次数: 0
           
作者中文名作者英文名单位中文名单位英文名E-Mail
褚皓天 CHU Hao-tian 呼吸疾病中医药防治省部共建协同创新中心,河南 郑州 450046 Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province & Education Ministry of P.R. China, Zhengzhou 450046, China  
赵鹏 ZHAO Peng 呼吸疾病中医药防治省部共建协同创新中心,河南 郑州 450046
河南中医药大学 中医药科学院,河南 郑州 450046
Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province & Education Ministry of P.R. China, Zhengzhou 450046, China
Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou 450046, China
 
田燕歌 TIAN Yan-ge 呼吸疾病中医药防治省部共建协同创新中心,河南 郑州 450046
河南中医药大学 中医药科学院,河南 郑州 450046
Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province & Education Ministry of P.R. China, Zhengzhou 450046, China
Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou 450046, China
 
任周新* REN Zhou-xin 呼吸疾病中医药防治省部共建协同创新中心,河南 郑州 450046
河南中医药大学 中医药科学院,河南 郑州 450046
Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province & Education Ministry of P.R. China, Zhengzhou 450046, China
Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou 450046, China
 
基金项目:国家自然科学基金项目(82074406);国家中医临床研究基地业务建设第二批科研专项项目(JDZX2015l56)
中文摘要:3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法具有操作简单和成本低廉等优点,被广泛应用于中药成分的细胞毒性和对细胞增殖影响等研究。然而,细胞的数量和密度、MTT浓度和作用时间等实验条件会干扰检测结果的准确性。另外,由于中药成分种类繁多而且化学结构复杂,可能导致吸收光谱漂移、形成近似检测波长的吸收峰及干扰细胞代谢的活性等,影响MTT检测结果的准确性。对近年的相关文献进行整理,归纳和分析了影响MTT实验的实验条件、优化措施和质量控制,归类了检测需要注意的某些中药成分,总结了替代MTT法的其他实验及MTT与其他实验的联合应用,对应用中存在的问题进行了探讨和展望,期望为MTT法在中药成分相关实验中的合理应用提供参考。
中文关键词:干扰因素  中草药成分  3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐法  优化措施  替代方法
 
Interference Factors and Countermeasures in MTT Assay for Detection of Chinese Herbal Medicine Components with Cytotoxicity and Influencing Cell Proliferation
Abstract:The methyl thiazolyl tetrazolium (MTT) assay with simple operation and low cost has been widely used in the research on the Chinese herbal medicine components which have cytotoxicity and influence cell proliferation. However, the test conditions such as the number and density of cells, MTT concentration, and reaction duration interfere with the accuracy of the test results. In addition, the diverse components with complex structures in Chinese herbal medicines may affect the accuracy of the detection results due to the drift of the absorption spectrum, interference of cellular metabolic activity, absorption peaks of approximate detection wavelengths, which result in erroneous conclusions. By reviewing the relevant articles published in recent years, the paper summarizes and analyzes the interference factors in test conditions and corresponding optimization measures and quality control measures. Furthermore, this paper classifies some Chinese herbal medicine components that need attention in the assay, summarizes the alternative methods of MTT assay and the application of MTT assay combined with other assays, and discusses the existing problems in the application, aiming to provide reference for the rational application of MTT assay in the research on Chinese herbal medicine components.
keywords:interference factors  Chinese herbal medicine components  MTT assay  optimization measures  alternative methods
查看全文   查看/发表评论  下载PDF阅读器