| Abstract:Objective : Based on psbA-trnH sequence, DNA barcoding technology was used to identify and analyze samples of Huangjing medicinal materials from 12 regions and 2 regions outside Liaoning Province, so as to provide a theoretical basis for the identification and genetic diversity analysis of Huangjing medicinal materials in Liaoning Province. Materials and methods: DNA was extracted from the medicinal sites of 38 xanthris samples by DNA kit extraction method, polymerase chain reaction (PCR) amplification of psbA-trnH fraction was performed for bidirectional sequencing, and different source and exogroup sequences of medicinal plant xanthin were downloaded from GenBank, the sequencing results were spliced by SeqMan software, the data were analyzed and compared using MEGA 11.0, and K2P (Kimura 2- parameter) genetic distance, and the phylogenetic tree was established using the adjacency method (NJ) for analysis. Results: According to the results of the NJ phylogenetic tree, all the samples of Huangjin from different sources were clustered into one large branch, and the outer group of pig tooth flowers were clustered into one, which was more obvious. Liaoning Province, Hubei Province and Guizhou Province Huangjing and NCBI downloaded Huangjing gathered into one, and combined with the mutation site and genetic distance, it can be seen that the Huangjing species in Liaoning Province, Hubei Province and Guizhou Province are Polygonatum sibiricum Red., and the interspecific differences are very small, and 4 samples collected in Liaoning Province have mutation sites, and the base sequences of the rest of the samples are the same. Conclusion: The DNA barcoding technology using psbA-trnH sequence can distinguish and identify Huangjing medicinal materials and their base plants of different origins with high success rate, which can be used for intraspecific and interspecific identification of Huangjing species, and provide a reference for ensuring the accurate identification of the source of Huangjing medicinal materials in Liaoning Province. |
