| 不同生长年限与采收季节对何首乌中14个成分含量的影响 |
| 投稿时间：2023-03-15 修订日期：2023-04-19 点此下载全文
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| 基金项目:] 国家自然科学基金资助项目(81973476) |
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| 中文摘要:目的 探究何首乌Polygoni Multiflori Radix随生长年限和采收季节的成分含量变化规律。方法 建立超高效液相色谱-三重四极杆质谱分析方法(UPLC-QQQ-MS/MS),测定德庆地区何首乌药材中14个成分含量。结果 建立了准确可靠的UPLC-QQQ-MS/MS测定何首乌中trans-THSG、cis-THSG、2,3,5,4’-四羟基二苯乙烯-2-O-(2”-O-阿魏酰)-β-D-吡喃葡萄糖苷、虎杖苷、白藜芦醇、儿茶素、表儿茶素、金丝桃苷、芦丁、没食子酸、对羟基苯甲酸、对羟基苯甲醛、对香豆酸和决明酮-8-O-β-D-葡萄糖苷含量的方法；随生长年限的增加,二苯乙烯苷类、黄酮类、酚类化合物含量多降低；随采收季节的变化,二苯乙烯苷类、黄酮类、决明酮-8-O-β-D-葡萄糖苷成分多春季含量最高、秋季含量最低,酚类化合物多夏季含量最高、春秋含量低。结论 成分的含量与药材生长年限及采收季节密切相关,其中采收季节变化较为明显；以trans-THSG、决明酮-8-O-β-D-葡萄糖苷作为潜在肝毒性物质,秋季含量低于春夏采收样品,为适宜采收期,符合药典规定。 |
| 中文关键词:何首乌 超高效液相色谱三重四级杆质谱(超高液相色谱-三重四极杆质谱仪 二苯乙烯苷类成分 黄酮类成分 酚类成分 采收季节 生长年限 |
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| Influences of growth years and harvest seasons on the content of 14 components in Polygoni Multiflori Radix |
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| Abstract:Objective To explore the change rule of component content of Polygoni Multiflori Radix (PM) with growth years and harvest season. Methods Ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS) was used to quantify 9 anthraquinone components in PM, including trans-THSG,cis-THSG,2,3,5,4"-tetrahydroxystilbene-2-O-(2"-O-A Fluoroyl)-β-D -glucopyranoside,Polygonin, Resveratrol, Catechin, Epicatechin, Hyperoside, Rutin, Gallic acid, p-Hydroxybenzoic acid, p-Hydroxybenzaldehyde, p-Coumaric acid, cassia-8-O-β-D -glucoside. The content of stilbene glycosides, flavonoids and phenolic compounds is found to decrease with the increase of growth years. Stilbene glycosides, flavonoids, cassia-8-O-β-D -glucoside was the highest in spring and the lowest in autumn, Phenolic was the highest in summer. Conclusion The content of components is closely related to the growth years and harvest seasons, the influence of harvesting seasons was greater than that of the cultivation years. It is considered that autumn is suitable for harvesting PM, the potential toxic substances trans-THSG and cassia-8-O-β-D -glucoside content is low, which is consistent with the provisions of the pharmacopoeia. |
| keywords:Polygoni Multiflori Radix UPLC-QQQ-MS/MS stilbene glycosides flavonoids phenols harvest season growth years |
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