| Abstract:[Abstract] Objective: To study the effect of gypsum decocting technology on the physicochemical properties and substance forms of Qing-Fei-Pai-Du-Tang particle system. Methods: The decoction of Qing-Fei-Pai-Du-Tang and the preparation of formula granules were prepared by different decoction methods of gypsum (direct decoction, first decoction, package decoction and other decoction). The solid particles in each decoction and formula granule preparation solution were separated by gradient centrifugation at centrifugal forces of 830, 3300 and 13200×g, and the particle size, potential, morphology, element distribution, protein and polysaccharide content were characterized. Results: The particle size of each decoction groups and granule groups decreased with the increase of centrifugal force, and the particle size of decoction groups was larger than that of granule groups. The potential of microsomes in each decoction groups and granule groups was always negative value but there was no significant difference. The preparation method of decoction and the decoction method of gypsum have significantly affected the morphology of the granules of Qing-Fei-Pai-Du-Tang: the decoction microsomes in gypsum direct decoction and other decoction groups showed round and ellipsoidal agglomeration shapes with different sizes, in which flaky and fibrous substances are dispersed; the decoction microsomes of gypsum first decocted and decocted groups showed reticular structure; the microsomes of granules show irregular bulk accumulation. The element composition of each microsome is C, O and Ca, and the polysaccharide content is higher than that of protein, but the content of polysaccharide in the decoction groups was lower than that in the formula granule preparation solution groups. Conclusion: The particle size, potential, morphology, element distribution, protein and polysaccharide content of Qing-Fei-Pai-Du-Tang formed by "Co-decocting" are different from those of the formula granule preparation solution, and the decocting technology of gypsum will also affect the physicochemical properties and substance forms of decoction granules. |
