| 三七药材中262种农药多残留筛查及风险评估研究 |
| 投稿时间：2023-08-30 修订日期：2023-11-19 点此下载全文
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| 中文摘要:目的 对文山三七中多农药残留进行筛查研究,初步了解其农药残留状况,并评估高检出农药的健康风险,为现有标准最大残留限量值的制修订提供科学依据。方法 对收集的16批次文山三七采用乙腈为溶媒,高速匀浆法提取处理。针对拟定的检测指标,分别采用气相串联质谱(GC-MS/MS)和高效液相串联质谱(LC-MS/MS),以多反应监测(MRM)模式进行检测,以不少于2组特征离子对进行定性,并针对有检出的农药,以校准曲线法进行定量检测。最后结合课题组前期建立的风险评估方法,对检出率较高的高风险农药进行暴露评估,计算急性和慢性摄入风险。结果 所建方法共有262种农药指标(总计271个化学单体),在线性范围内线性关系良好,灵敏度、回收率均符合痕量多残留分析技术要求。16批三七样品中共检出50种农药,农药检出率较高,多数检出农药处于痕量水平,仅百菌清、毒死蜱、三氟氯氰菊酯、异菌脲、五氯硝基苯、甲基硫菌灵等超出团体标准(T/CATCM003-2017)的最大残留限量(MRL)。此外,水洗可明显降低部分检出农药的残留量。对超出限量标准及无相关限量标准但检出率较高的农药品种进行风险评估,结果9种农药的短期危害指数(HIa)在0.000 2～0.141 8,长期危害指数(HIc)在0.000 4～0.009,其中五氯硝基苯的短期危害指数达到0.141 8。长期危害指数也最高,达到0.009,但均低于１,尚处于可接受风险。结论 本研究建立了三七药材中262种农药多残留筛查方法,并采用暴露评估之点评估模式对其进行健康风险评估,可为完善三七药材相关农药残留限量标准的制修订提供技术参考和依据。 |
| 中文关键词:三七 农药多残留 气相色谱串联质谱法 液相色谱串联质谱法 风险评估 危害指数 残留限量标准 |
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| Study on screening and risk assessment of 262 pesticides in Notoginseng Radix |
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| Abstract:Abstract： Objective: To screen and assess the levels of multiple pesticide residues in Panax Notoginseng, and evaluate the intake exposure of the highly detected pesticide residues. And provide a scientific basis for the revision of the existing standard maximum residue limits.
Methods: 16 batches of yunnan wenshan Panax Notoginseng samples were extracted by high speed homogenate with acetonitrile as solvent．And their residues were analyzed by GC-MS /MS and LC-MS /MS in MRM mode．No less than 2 groups of characteristic ion pairs were adopted for qualitative detection，and the calibration curve method was used for quantitative detection．Finally, combined with the risk assessment method established by the research group, the exposure assessment of high-risk pesticides with high detection rate was carried out. The point assessment model was used to calculate the acute and chronic ingestion risks. Results: Totally 262 pesticides (involving 270 chemical monomers) with monitoring significance to traditional Chinese medicinal materials were detected．The established method had good linear relationship in the linear range, sensitivity and recovery rate met the technical requirements of trace multi-residue analysis. The results showed that 50 pesticides were detected in 16 batches of samples，the detection rate of pesticide was higher. but most of the detected pesticides were at the trace level，only chlorothalonil, chlorpyrifos, cyfluthrin, isometrin, pentachloronitrobenzene, methyl thiobacillin exceeded the group standard （T/CATCM003-2017）maximum residue limits (MRL). In addition, water washing could significantly reduce the residual amount of some detected pesticides. Risk assessment was carried out for pesticide varieties exceeding MRL or pesticide with high detection rate. The results shown that the short-term hazard index (HIa) and long-term hazard index (HIc) of 9 pesticides were 0.0002 ~ 0.1418 and 0.0004 ~ 0.009 respectively. Among them the HIc of pentachloronitrobenzene was 0.1418. The HIa was 0.009, which was also the highest. As the values were below 1, it was indicated that the threat of this pesticide in Notoginseng Radix to human health is acceptable. Conclusion: In this study, 262 pesticide multi-residue screening methods in Panax Notoginseng were established, and the health risk assessment was conducted by using the point assessment model of exposure assessment, which could provide technical support for improving the revision of pesticide residue limit standards in Panax notoginseng. |
| keywords:Notoginseng Radix multiple pesticide residues gas chromatography tandem mass spectrometry liquid chromatography tandem mass spectrometry risk assessment Hazard index residue limited standards |
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