基于HPLC指纹图谱和网络药理学探究连翘保肝作用潜在机制
投稿时间:2024-06-06  修订日期:2024-06-24   点此下载全文
引用本文:
DOI:
摘要点击次数: 153
全文下载次数: 0
                 
作者中文名作者英文名单位中文名单位英文名E-Mail
田溪 tianxi 河北省农林科学院经济作物研究所 Economic Crop Research Institute of Hebei Academy of Agriculture and Forestry 864559367@qq.com 
李雯钰 Li Wenyu 河北医科大学药学院 School of Pharmacy, Hebei Medical University 1064789969@qq.com 
张惠怡 Zhang Huiyi 河北医科大学药学院 School of Pharmacy, Hebei Medical University 1156507919@qq.com 
李红艳 Li Hongyan 河北省农林科学院经济作物研究所 Economic Crop Research Institute of Hebei Academy of Agriculture and Forestry 57946481@qq.com 
刘灵娣 Liu Lingdi 河北省农林科学院经济作物研究所 Economic Crop Research Institute of Hebei Academy of Agriculture and Forestry nkyliulingdi@126.com 
温春秀* Wen Chunxiu 河北省农林科学院经济作物研究所 Economic Crop Research Institute of Hebei Academy of Agriculture and Forestry wenchunxiu@126.com 
中文摘要:目的 根据HPLC指纹图谱和网络药理学探讨连翘保肝作用的药效关联物质及作用机制。方法 采用HPLC建立 28 批连翘指纹图谱,结合对照品指认特征峰,进一步结合相似度评价、聚类分析和偏最小二乘判别分析进行评价,筛选药效关联物质,进行网络药理学分析。利用TCMIP、Swiss Target Prediction数据库检索成分靶点;利用OMIM、GeneCards和Drugbank数据库检索肝损伤疾病靶点,取成分与疾病的交集靶点,采用String数据库构建蛋白质-蛋白质相互作用网络图,并进行拓扑学参数分析;采用Cytoscape 3.8.2软件构建“成分-疾病-靶点”网络图;采用Metascape网站对交集靶点进行GO和KEGG富集分析。结果 连翘 HPLC 指纹图谱的相似度均>0.90,通过聚类分析将 28 批样品聚为 3 类,偏最小二乘判别分析结果与聚类分析结果一致。经对照品比对及综合分析,确定连翘酯苷E、松脂醇二葡萄糖苷、连翘酯苷A、异槲皮苷、连翘苷为连翘的药效关联物质,其可能通过调控 HRAS、PTGS2、MCL1、IL2、HSP90AA1 等核心靶点及 IL-17 信号通路、花生四烯酸代谢信号通路、PI3K-Akt 信号通路等关键信号通路来发挥作用。结论 本研究结合指纹图谱及网络药理学,对连翘保肝作用药效关联物质及作用机制进行了探讨,为连翘的质量控制、品种选育及临床研究提供参考。
中文关键词:连翘  HPLC指纹图谱  网络药理学  肝损伤保护
 
Exploring the potential mechanism of liver injury protection of Forsythia suspensa based on HPLC fingerprint and network pharmacology
Abstract:Objective: To xplore the pharmacological substances and mechanisms of action related to the protective effect of Forsythia suspensa on liver injury based on HPLC fingerprint and network pharmacology. Method: 28 batches of Forsythia suspensa fingerprint were established using HPLC, and the characteristic peaks were identified by reference materials. Further evaluation was conducted by combining similarity evaluation, cluster analysis, and partial least squares discriminant analysis to screen for drug related substances and conduct network pharmacology analysis. Component related targets were retrieved using TCMIP and Swiss Target Prediction databases. Liver injury related targets were obtained using OMIM, GeneCards, and Drugbank databases. Intersection targets were selected between components and diseases, PPI network diagram was constructed, and topological analysis was performed using the String database and Cytoscape 3.8.2. Cytoscape 3.8.2 software was used to construct a "component disease target" network diagram using. GO and KEGG enrichment analysis were performed on intersection targets using the Metascape website. The similarity of HPLC fingerprints of Forsythia suspensa was all greater than 0.90, and 28 batches of samples were clustered into 3 categories through cluster analysis. The results of partial least squares discriminant analysis were consistent with those of cluster analysis. Through comparison and comprehensive analysis of reference materials, it has been determined that forsythoside E, pinoresinol diglucoside, forsythoside A, isoquercitrin, phillyrin were drug related substances. They may exert their effects by regulating core targets such as HRAS, PTGS2, MCL1, IL2, HSP90AA1, as well as key signaling pathways such as IL-17 signaling pathway, arachidonic acid metabolism signaling pathway, and PI3K-Akt signaling pathway. Conclusion: This study combines fingerprint and network pharmacology to explore the pharmacological substances and mechanisms associated with the protective effect of Forsythia suspensa on liver injury, providing reference for quality control, variety selection, and clinical research of Forsythia suspensa.
keywords:Forsythia suspensa  HPLC fingerprint  Network pharmacology  Liver injury protection
查看全文   查看/发表评论  下载PDF阅读器