| 基于HPLC指纹图谱的葶苈大枣泻肺汤相关评价 |
| 投稿时间：2023-04-17 点此下载全文
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| 引用本文：马云,关永霞,王艳,严琴琴,周冰,申凤霞,李倩,聂宗恒,范建伟,赵利枝.基于HPLC指纹图谱的葶苈大枣泻肺汤相关评价[J].中国现代中药,2024,26(2):342-347 |
| DOI：10.13313/j.issn.1673-4890.20230417001 |
| 摘要点击次数: 254 |
| 全文下载次数: 0 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 马云 |
MA Yun |
鲁南制药集团股份有限公司 经方与现代中药融合创新全国重点实验室，山东 临沂 276006
鲁南厚普制药有限公司 中药制药共性技术山东省工程研究中心，山东 临沂 276006 |
Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Linyi 276006, China
Lunan Hope Pharmaceutical Co., Ltd., Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine, Linyi 276006, China |
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| 关永霞 |
GUAN Yong-xia |
鲁南制药集团股份有限公司 经方与现代中药融合创新全国重点实验室，山东 临沂 276006
鲁南厚普制药有限公司 中药制药共性技术山东省工程研究中心，山东 临沂 276006 |
Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Linyi 276006, China
Lunan Hope Pharmaceutical Co., Ltd., Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine, Linyi 276006, China |
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| 王艳 |
WANG Yan |
临沂大学，山东 临沂 276000 |
Linyi University, Linyi 276000, China |
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| 严琴琴 |
YAN Qin-qin |
鲁南制药集团股份有限公司 经方与现代中药融合创新全国重点实验室，山东 临沂 276006
鲁南厚普制药有限公司 中药制药共性技术山东省工程研究中心，山东 临沂 276006 |
Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Linyi 276006, China
Lunan Hope Pharmaceutical Co., Ltd., Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine, Linyi 276006, China |
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| 周冰 |
ZHOU Bing |
鲁南制药集团股份有限公司 经方与现代中药融合创新全国重点实验室，山东 临沂 276006
鲁南厚普制药有限公司 中药制药共性技术山东省工程研究中心，山东 临沂 276006 |
Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Linyi 276006, China
Lunan Hope Pharmaceutical Co., Ltd., Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine, Linyi 276006, China |
|
| 申凤霞 |
SHEN Feng-xia |
鲁南制药集团股份有限公司 经方与现代中药融合创新全国重点实验室，山东 临沂 276006
鲁南厚普制药有限公司 中药制药共性技术山东省工程研究中心，山东 临沂 276006 |
Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Linyi 276006, China
Lunan Hope Pharmaceutical Co., Ltd., Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine, Linyi 276006, China |
|
| 李倩 |
LI Qian |
鲁南制药集团股份有限公司 经方与现代中药融合创新全国重点实验室，山东 临沂 276006
鲁南厚普制药有限公司 中药制药共性技术山东省工程研究中心，山东 临沂 276006 |
Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Linyi 276006, China
Lunan Hope Pharmaceutical Co., Ltd., Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine, Linyi 276006, China |
|
| 聂宗恒 |
NIE Zong-heng |
鲁南制药集团股份有限公司 经方与现代中药融合创新全国重点实验室，山东 临沂 276006
鲁南厚普制药有限公司 中药制药共性技术山东省工程研究中心，山东 临沂 276006 |
Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Linyi 276006, China
Lunan Hope Pharmaceutical Co., Ltd., Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine, Linyi 276006, China |
|
| 范建伟* |
FAN Jian-wei |
鲁南制药集团股份有限公司 经方与现代中药融合创新全国重点实验室，山东 临沂 276006
鲁南厚普制药有限公司 中药制药共性技术山东省工程研究中心，山东 临沂 276006 |
Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Linyi 276006, China
Lunan Hope Pharmaceutical Co., Ltd., Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine, Linyi 276006, China |
|
| 赵利枝* |
ZHAO Li-zhi |
鲁南制药集团股份有限公司 经方与现代中药融合创新全国重点实验室，山东 临沂 276006
鲁南厚普制药有限公司 中药制药共性技术山东省工程研究中心，山东 临沂 276006 |
Lunan Pharmaceutical Group Co., Ltd., State Key Laboratory of Integration and Innovation of Classic Formula and Modern Chinese Medicine, Linyi 276006, China
Lunan Hope Pharmaceutical Co., Ltd., Shandong Engineering Research Center of Generic Manufacture Technology of Traditional Chinese Medicine, Linyi 276006, China |
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| 基金项目:山东省重大科技创新工程（2018CXGC1305）；山东省中医经典名方协同创新中心开放课题（2018KFY02） |
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| 中文摘要:目的 建立葶苈大枣泻肺汤物质基准的高效液相色谱法（HPLC）指纹图谱和6个指标成分的含量测定方法。方法 采用反相高效液相色谱梯度洗脱法，借助“中药色谱指纹图谱相似度评价系统”（2012A版）建立14批葶苈大枣泻肺汤的HPLC特征指纹图谱，同时测定槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷、芦丁、异槲皮苷、异鼠李素-3-O-β-D-葡萄糖苷、槲皮素和异鼠李素6个指标成分的含量。结果 葶苈大枣泻肺汤的HPLC特征指纹图谱共标定了27个共有峰，6个特征峰分别为5号峰（槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷）、18号峰（芦丁）、19号峰（异槲皮苷）、21号峰（异鼠李素-3-O-β-D-葡萄糖）、24号峰（槲皮素）和27号峰（异鼠李素）。对14批葶苈大枣泻肺汤进行含量测定，槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷、芦丁、异槲皮苷、异鼠李素-3-O-β-D-葡萄糖苷、槲皮素和异鼠李素质量浓度分别为111.33~118.71、9.16~9.36、16.51~17.26、12.23~12.62、3.10~3.36、3.51~3.62 μg·mL–1，方法精密度、稳定性、重复性均良好。结论 建立的HPLC指纹图谱和多指标成分含量测定方法稳定可靠，可为葶苈大枣泻肺汤的质量标准研究提供参考。 |
| 中文关键词:葶苈大枣泻肺汤 物质基准 高效液相色谱法 指纹图谱 含量测定 |
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| Evaluation of Tingli Dazao Xiefei Decoction Based on HPLC Fingerprint Technology |
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| Abstract:Objective To establish the high-performance liquid chromatography (HPLC) fingerprint of Tingli Dazao Xiefei Decoction and a method for determination of six index components.Method The HPLC fingerprints of 14 batches of Tingli Dazao Xiefei Decoction were established by reverse-phase HPLC gradient elution method according to the similarity evaluation system for chromatographic fingerprint of Traditional Chinese Medicine (Version 2012A). At the same time, the contents of six index components (quercetin-3-O-β-D-glucose-7-O-β-D-gentiobiglycoside, rutin, isoquercitrin, isorhamnetin-3-O-β-D-glucoside, quercetin and isorhamnetin) were determined.Results A total of 27 common peaks were calibrated according to the HPLC characteristic fingerprints of Tingli Dazao Xiefei Decoction, and six chromatographic peaks were identified, including peak 5 (quercetin-3-O-β-D-glucose-7-O-β-D-gentiobiglycoside), peak 18 (rutin), peak 19 (isoquercitrin), peak 21 (isorhamnetin-3-O-β-D-glucose), peak 24 (quercetin), and peak 27 (isorhamnetin). The content determination for the 14 batches of Tengli Dazao Xiefei Decoction resulted in the following mass concentrations. The content of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobiglycoside was 111.33-118.71 μg·mL–1, the content of rutin was 9.16-9.36 μg·mL–1, the content of isoquercitrin was 16.51-17.26 μg·mL–1, the content of isorhamnetin-3-O-β-D-glucoside was 12.23-12.62 μg·mL–1, and the content of quercetin was 3.10-3.36 μg·mL–1, and the content of isorhamnetin was 3.51-3.62 μg·mL–1. The established fingerprint and content determination method exhibited good precision, stability and reproducibility.Conclusion The established HPLC fingerprint profile and multi-index component quantification method are stable and reliable, which can provide a reference for the study on the material basis and quality standard of Tingli Dazao Xiefei Decoction. |
| keywords:Tingli Dazao Xiefei Decoction material standard HPLC fingerprint content determination |
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