| Abstract:Objective: To reveal the effect and essence of ginseng rusty root symptoms on ginseng quality. Methods: the phloem tissue of healthy ginseng (AG), phloem tissue around red-patches of rusty ginseng (BG), and red-patches tissue of rusty ginseng (CG) were collected，to detected the contents of O2?-, H2O2, and MDA, the activities of antioxidant enzymes (SOD, CAT and POD) and secondary metabolism-related enzymes (HMGCR, FPS, SS, SE, DE and DS). The contents of ginsenosides Rg1, Re, Rf, Rb1, Rc, Ro, Rb2, Rb3 in healthy ginseng and rusty ginseng were analyzed by HPLC. Result: The test results of the ginseng samples in different months showed the same change trend. Compared with the AG group, the contents of ROS, the activities of antioxidant enzymes and secondary metabolize-related enzymes in the BG group reached the highest, and the MDA content in the CG group reached the highest. Compared with healthy ginseng, the contents of ginsenosides significantly increased in the early stage of rusty ginseng in June and significantly decreased in the late stage in August. Conclusion: Ginseng rusty root symptoms can affect ginseng quality, with higher levels of saponins in the early stages of the disease or under less stressful conditions, and lower in the severe conditions or the later stages of the disease. |
