| Abstract:Objective: The quality of Andrographis paniculata from different countries and regions was analyzed by fingerprint, chemical pattern recognition and multi-component content measurement. Methods: Through the determination of the characteristic spectrum of 26 batches of A. paniculata, the HPLC fingerprints of A. paniculata were established and the common peaks were identificated by reference substances. Similarity evaluation and principal component analysis (PCA) were carried out on the fingerprints and orthogonal partial least squares discriminant analysis (OPLS-DA) was used to find the different components of A. paniculata from different producing areas. Results: There were 15 common peaks in the fingerprints of A. paniculata, except for the three batches of samples S2, S4, and S15, the similarity of the other 23 batches was greater than 0.8. According to the analysis of PCA, 26 batches of A. paniculata were divided into two categories; Three biomarkers were determined by OPLS-DA method, namely andrographolide, 14-deoxyandrographolide and dehydroandrographolide, respectively. The contents of diterpene lactone in A. paniculata from different areas at home and abroad were significantly different. Conclusion: The established fingerprint method combined with chemical pattern recognition can effectively analyze the quality difference of A. paniculata from different producing areas, and the diterpene lactone content of andrographis paniculata from different producing areas at home and abroad is significantly different, which provides a reference for the quality and planting of A. paniculata. |
