| 基于化学计量法和多成分定量分析的断血流不同药用部位及产地质量评价 |
| 投稿时间：2024-04-07 修订日期：2024-06-18 点此下载全文
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| 中文摘要:目的 建立断血流的特征图谱和含量测定方法,通过化学计量法评价不同产地和不同药用部位的质量。方法 采用UPLC法,应用Aglient SB C18色谱柱(2.1 mm×100 mm,1.8 μm)；流动相为乙腈(A)和0.1%甲酸水溶液(B),梯度洗脱,流速0.3 mL.min-1；柱温30 ℃；进样体积3 μL；检测波长：263 nm。运用多组分检验柱状图、主成分分析、正交偏最小二乘判别等分析方法综合评价断血流不同地区和药用部位的质量,并采取数据可视化制作植物热图进行直接表征。结果 建立的特征图谱对断血流中13个共有成分识别,经方法学验证所建方法适用于断血流药材的特征图谱及含量测定；除DXL001、007外,21批样品的相似度为0.877～0.99。不同药用部位经PCA、OPLS-DA等分析方法可明显分为两类,得到六个差异性标物质,表明茎、叶部位的成分含量差异显著；不同成分含量累计柱状图表明在不同地区中以安徽的质量最佳。结论 断血流特征图谱和含量测定方法科学准确,合理可行；表明不同产地和不同药用部位的质量具有显著差异性,叶部位质量明显高于茎部位,为断血流的质量评判提供了理论依据。 |
| 中文关键词:断血流 化学计量法 UPLC 特征图谱 含量测定 不同产地 不同药用部位 质量评价 |
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| Quality evaluation of different medicinal parts and origins of Clinopodium herba based on chemometrics and quantitative multi-component analysis |
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| Abstract:Objective To establish a characterization profile and content determination method for Clinopodii herba, and to evaluate the quality of Clinopodii herba from different origins and medicinal parts using chemometric methods. Methods Ultra-Performance Liquid Chromatography (UPLC) was performed on an Agilent SB C18 column (2.1 mm × 100 mm, 1.8 μm) using mobile phases of acetonitrile (A) and 0.1% formic acid aqueous solution (B) in gradient elution mode at a flow rate of 0.3 mL/min. The detection wavelength was set at 263 nm, with an injection volume of 3 μL and a column temperature of 30°C. Principal component analysis (PCA), orthogonal partial least squares discrimination analysis (OPLS-DA), and other analytical methods were employed to comprehensively evaluate the quality of different regions and medicinal parts of Clinopodii herba. Data visualization techniques were used to produce botanical thermograms for direct characterization. Results The established characterization maps identified 13 common components in Clinopodii herba. The methods were validated as suitable for feature mapping and content determination. The similarity among 21 batches of samples, except for DXL001 and 007, ranged from 0.877 to 0.99. PCA and OPLS-DA classified the different medicinal parts into two distinct categories, yielding six differentiated markers. This classification indicated that the stem and leaf parts could be divided into two groups. Cumulative histograms of the contents of different components revealed that the quality of samples from Anhui was superior among the regions studied. Conclusion The characterization and content determination methods are scientific, accurate, reasonable, and feasible. They demonstrate significant differences in quality between different origins and medicinal parts, with the leaf part exhibiting notably higher quality than the stem part. These findings provide a theoretical basis for the quality assessment of Clinopodii herba. |
| keywords:Clinopodium chinensis Chemometry UPLC Characteristic map Content determination Different origin Different medicinal parts Quality evaluation |
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