| Abstract:Objective: to study the effect of different growth time and ecological factors on the content of Phillyrin in Forsythia suspensa leaves. Methods: Forsythia suspensa leaves were collected from different altitudes and slopes in Lingchuan County of Shanxi province. The leaves were analyzed by using SEMERFEL U3000 liquid chromatography with shim-packgis C18 column (250 mm × 4.6 mm, 5 μm) , the content of Phillyrin in Forsythia suspensa leaves was determined dynamically, and statistical analysis was performed with SPSS 24.0 software. One way ANOVA method and Duncan multiple comparison method were used, the effects of ecological factors such as growth time, altitude and slope direction on Phillyrin content in Forsythia suspensa leaves were analyzed. Results: 1) growth time was the main factor affecting the content of Forsythiaside in Forsythia suspensa leaves. The content of Forsythiaside in Forsythia suspensa leaves varied with different growth time. The content of Forsythiaside in Forsythia suspensa leaves reached the highest value (4.38%) from late June to early and middle July, then gradually decreased, reaching the lowest value (0.64%) at the end of October. 2) in the region of 800m-1300m altitude, the content of Forsythiaside in leaves gradually increased with the increase of altitude, reaching the highest at 1300m altitude (average 3.27%) . Subsequently, the content of Forsythiaside in leaves gradually decreased with the increase of altitude. 3) slope orientation had statistical significance on the content of Forsythiaside in leaves (p < 0.05) , and the content of Forsythiaside slopes and sunny slopes was the highest. 4) soil layer thickness had statistical significance on the content of Forsythiaside in leaves (p < 0.05) , and soil layer thickness of soil was the highest in soil 51cm. 5) there was no statistical significance was found in the content of forsythiaside from different tree species. Conclusion: the content of Phillyrin in Forsythia suspensa leaves is affected by growth time, altitude, slope direction and soil layer thickness. |
