| 基于UPLC-Q-TOF-MS/MS的“略麻1号”茎秆与块茎化学成分分析 |
| 投稿时间：2024-01-11 点此下载全文
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| 引用本文：李炳佳,贺文文,白吉庆,王小平,李军茂,李晓东.基于UPLC-Q-TOF-MS/MS的“略麻1号”茎秆与块茎化学成分分析[J].中国现代中药,2024,26(5):788-798 |
| DOI：10.13313/j.issn.1673-4890.20240111002 |
| 摘要点击次数: 146 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 李炳佳 |
LI Bing-jia |
江西中医药大学，江西 南昌 330000
陕西省中药原料质量监测技术服务中心，陕西 咸阳 712046 |
Jiangxi University of Chinese Medicine, Nanchang 330000, China
Shaanxi Provincial Quality Monitoring and Technical Service Center for Chinese Medicinal Materials, Xianyang 712046, China |
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| 贺文文 |
HE Wen-wen |
陕西中医药大学，陕西 咸阳 712046 |
Shaanxi University of Chinese Medicine, Xianyang 712046, China |
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| 白吉庆* |
BAI Ji-qing |
陕西省中药原料质量监测技术服务中心，陕西 咸阳 712046
陕西中医药大学，陕西 咸阳 712046 |
Shaanxi Provincial Quality Monitoring and Technical Service Center for Chinese Medicinal Materials, Xianyang 712046, China
Shaanxi University of Chinese Medicine, Xianyang 712046, China |
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| 王小平 |
WANG Xiao-ping |
陕西中医药大学，陕西 咸阳 712046 |
Shaanxi University of Chinese Medicine, Xianyang 712046, China |
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| 李军茂 |
LI Jun-mao |
江西中医药大学，江西 南昌 330000 |
Jiangxi University of Chinese Medicine, Nanchang 330000, China |
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| 李晓东 |
LI Xiao-dong |
略阳县中药材产业发展中心，陕西 汉中 724300 |
Lueyang County Traditional Chinese Medicine Industry Development Center, Hanzhong 724300, China |
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| 基金项目:陕西省中医药管理局“秦药”优良种苗选育项目 |
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| 中文摘要:目的 对“略麻1号”茎秆与块茎化学成分进行比较研究。方法 采用超高效液相色谱-四极杆飞行时间质谱法（UPLC-Q-TOF-MS/MS），Xbridge BEH C18色谱柱（100 mm×2.1 mm，1.7 μm），以0.1%甲酸水溶液-乙腈为流动相进行梯度洗脱，流速为0.3 mL·min–1；扫描方式采用正、负离子Full-ddMS2模式下采集数据；结合对照品、相关文献及质谱裂解规律对样品中成分的二级质谱数据进行分析。结果 从“略麻1号”茎秆中共鉴定出153个化合物，包括酚类20个、有机酸类46个、黄酮类11个、氨基酸类12个、生物碱类14个、其他类50个；从“略麻1号”块茎中共鉴定出126个化合物，包括酚类21个、有机酸类36个、氨基酸类13个、生物碱类11个、其他类45个。结论 “略麻1号”茎秆和块茎在物质基础上差异较大，在开发应用中应予以区别，茎秆具有较高的开发应用价值。 |
| 中文关键词:超高效液相色谱-四极杆飞行时间质谱法 天麻茎秆 化学成分 质谱裂解规律 酚类 |
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| Analysis of Chemical Components in Stem and Tuber of Gastrodia elata Bl. f. elata "Luema No. 1" by UPLC-Q-TOF-MS/MS |
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| Abstract:Objective To determine and compare the chemical components in the stem and tuber of Gastrodia elata Bl. f. elata "Luema No. 1".Methods Ultra-performance liquid chromatography-quadrupole/time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS) was performed with the Xbridge BEH C18 column (100 mm×2.1 mm,1.7 μm) and the mobile phases of 0.1% formic acid aqueous solution-acetonitrile. The gradient elution was conducted at a flow rate of 0.3 mL·min–1, and the data were collected by Full-ddMS2 in positive and negative ion modes. The reference substances,related literature, and mass spectrometry (MS) fragmentation patterns were taken as reference to analyze the MS/MS data of the components in the samples.Results A total of 153 compounds were identified in the stem of "Luema No. 1", including 20 phenols, 46 organic acids, 11 flavonoids, 12 amino acids, 14 alkaloids, and 50 other compounds. A total of 126 compounds were identified in the tuber of "Luema No. 1", including 21 phenols, 36 organic acids, 13 amino acids, 11 alkaloids, and 45 other compounds.Conclusion The stem and tuber of "Luema No. 1" showed different material bases and should be distinguished in the development and application. The stem demonstrates higher development and application value. |
| keywords:UPLC-Q-TOF-MS/MS stem of Gastrodia elata Bl. f. elata chemical component mass spectrometry fragmentation rules phenols |
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