| 3，6ʹ-二芥子酰基蔗糖神经保护作用研究 |
| 投稿时间：2024-02-20 点此下载全文
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| 引用本文：郑晓春,唐晓丽,刘雅楠,邢瀚文,印艳萍,方芳.3，6ʹ-二芥子酰基蔗糖神经保护作用研究[J].中国现代中药,2024,26(5):807-814 |
| DOI：10.13313/j.issn.1673-4890.20240220001 |
| 摘要点击次数: 134 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 郑晓春 |
ZHENG Xiao-chun |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 唐晓丽 |
TANG Xiao-li |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 刘雅楠 |
LIU Ya-nan |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 邢瀚文 |
XING Han-wen |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 印艳萍 |
YIN Yan-ping |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 方芳* |
FANG Fang |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 基金项目:国家自然科学基金面上项目（81373995）；北京中医药大学校级科研发展基金项目（BUCM-2021-HXFZJJ-010） |
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| 中文摘要:目的 研究远志中寡糖酯类成分3,6?-二芥子酰基蔗糖（DISS）对神经的保护作用。方法 将不同浓度的DISS与APP-SH-SY5Y细胞共同孵育24 h，四甲基偶氮唑盐（MTT）法检测各组细胞的存活率，免疫荧光法检测细胞内活性氧（ROS）水平，生化法检测超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性，ELISA法检测细胞内和培养液中β-淀粉样蛋白（Aβ）含量，蛋白质免疫印迹法（Western blot）检测细胞内淀粉样前体蛋白（APP）、微管相关蛋白轻链3-Ⅰ（LC3-Ⅰ）和LC3-Ⅱ的表达。结果 DISS可以提高APP-SH-SY5Y细胞的存活率（P<0.05），减少细胞内ROS的生成（P<0.01），提高SOD和GSH-Px活力（P<0.05），降低细胞内和培养液中的Aβ含量（P<0.01，P<0.05），明显升高细胞内LC3Ⅱ蛋白表达水平，升高LC3Ⅱ/LC3Ⅰ水平（P<0.05），对APP表达无明显影响。结论 DISS可能通过降低细胞内氧化应激，减少细胞Aβ表达及调节自噬发挥神经保护作用。 |
| 中文关键词:3,6ʹ-二芥子酰基蔗糖 APP-SH-SY5Y细胞 阿尔茨海默病 β淀粉样蛋白 氧化应激 自噬 |
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| Studies on Neuroprotective Effects of 3,6'-Disinapoyl Sucrose |
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| Abstract:Objective To study the neuroprotective effects of 3,6'-disinapoyl sucrose (DISS), an oligosaccharide ester component of the traditional Chinese medicine Polygala tenuifolia Willd.Methods Different concentrations of DISS were co-incubated with APP-SH-SY5Y cells for 24 h. The survival rate was detected by methyl thiazole tetrazolium (MTT) assay, The intracellular reactive oxygen species (ROS) levels were detected by immunofluorescence. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were detected by biochemical methods. The intracellular and culture medium contents of β-amyloid protein (Aβ) level were detected by enzyme-linked immunosorbent assay (ELISA). The expression of amyloid precursor protein (APP), microtubule-associated protein lightchain 3-Ⅰ (LC3-Ⅰ) and LC3-Ⅱ in cells were detected by western blotting.Results DISS could increase the viability of APP-SH-SY5Y cells (P<0.05), decrease the production of intracellular ROS (P<0.01), and increase the activities of SOD and GSH-px in APP-SH-SY5Y cells (P<0.05). Meanwhile, DISS also decreased the Aβ content in APP-SH-SY5Y cells (P<0.01) and cell culture medium (P<0.05), significantly increased the expression level of intracellular LC3Ⅱ protein and increased the ratio of LC3Ⅱ/LC3Ⅰ (P<0.05). However, DISS had no significant effect on APP expression.Conclusion DISS may exert neuroprotective effects by reducing intracellular oxidative stress, decreasing cellular Aβ expression and regulating autophagy. |
| keywords:3,6'-disinapoyl sucrose APP-SH-SY5Y cells Alzheimer's disease β-amyloid protein oxidative stress autophagy |
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