| 经典名方羌活胜湿汤指纹图谱建立及功效物质预测分析 |
| 投稿时间：2023-12-25 点此下载全文
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| 引用本文：姚子豪,熊富良,刘霞,张雪琼,汪沁柔,颜莉娟,杨元喜,陶正恒,李心愿.经典名方羌活胜湿汤指纹图谱建立及功效物质预测分析[J].中国现代中药,2024,26(5):815-822 |
| DOI：10.13313/j.issn.1673-4890.20231225003 |
| 摘要点击次数: 153 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 姚子豪 |
YAO Zi-hao |
武汉理工大学 化学化工与生命科学学院，湖北 武汉 430070 |
School of Chemistry, Chemical Engineering, and Life Sciences, Wuhan University of Technology, Wuhan 430070, China |
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| 熊富良 |
XIONG Fu-liang |
武汉理工大学 化学化工与生命科学学院，湖北 武汉 430070 |
School of Chemistry, Chemical Engineering, and Life Sciences, Wuhan University of Technology, Wuhan 430070, China |
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| 刘霞 |
LIU Xia |
武汉理工大学 化学化工与生命科学学院，湖北 武汉 430070 |
School of Chemistry, Chemical Engineering, and Life Sciences, Wuhan University of Technology, Wuhan 430070, China |
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| 张雪琼 |
ZHANG Xue-qiong |
武汉理工大学 化学化工与生命科学学院，湖北 武汉 430070 |
School of Chemistry, Chemical Engineering, and Life Sciences, Wuhan University of Technology, Wuhan 430070, China |
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| 汪沁柔 |
WANG Qin-rou |
武汉理工大学 化学化工与生命科学学院，湖北 武汉 430070 |
School of Chemistry, Chemical Engineering, and Life Sciences, Wuhan University of Technology, Wuhan 430070, China |
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| 颜莉娟 |
YAN Li-juan |
武汉理工大学 化学化工与生命科学学院，湖北 武汉 430070 |
School of Chemistry, Chemical Engineering, and Life Sciences, Wuhan University of Technology, Wuhan 430070, China |
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| 杨元喜 |
YANG Yuan-xi |
湖北太子药业有限公司，湖北 荆门 431800 |
Hubei Taizi Pharmaceutical Co., Ltd., Jingmen 431800, China |
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| 陶正恒 |
TAO Zheng-heng |
湖北太子药业有限公司，湖北 荆门 431800 |
Hubei Taizi Pharmaceutical Co., Ltd., Jingmen 431800, China |
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| 李心愿* |
LI Xin-yuan |
湖北省药品监督管理局检查中心，湖北 武汉 430077 |
Hubei Province Drug Administration Inspection Center, Wuhan 430077, China |
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| 基金项目:湖北省科技服务人才专项（2023DJC132） |
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| 中文摘要:目的 基于网络药理学方法和指纹图谱，分析预测经典名方羌活胜湿汤（QSD）的质量标志物（Q-marker）。方法 采用高效液相色谱法建立QSD指纹图谱，对共有峰进行归属和指认，并基于可测性和可溯源性筛选活性成分；运用网络药理学筛选靶点和通路，构建QSD的成分-靶点-通路相互作用网络，预测QSD的Q-marker。结果 共标定了20个共有峰，通过对照品指认出升麻素苷、洋川芎内酯Ⅰ、蛇床子素、紫花前胡苷、甘草酸铵、藁本内酯、阿魏酸、异欧前胡素、5-O-甲基维斯阿米醇苷、二氢欧山芹醇当归酸酯10个色谱峰，相似度均大于0.862；采用网络药理学方法对6个活性成分进行成分-靶点-通路网络构建与分析，富集的通路包括癌症中的蛋白聚糖、癌症的途径、化学致癌作用-受体激活、内分泌抵抗等。根据成分、靶点及通路之间的连接度初步预测二氢欧山芹醇当归酸酯、升麻素苷、甘草酸铵、异欧前胡素、蛇床子素、5-O-甲基维斯阿米醇苷6个活性成分可能通过调节这些信号通路达到祛风、胜湿和止痛的作用。结论 通过建立QSD指纹图谱，基于网络药理学方法分析预测QSD中的Q-marker，进一步明确QSD质量控制标准，有利于提高其质量控制水平，也为后期深入研究QSD的作用机制提供参考。 |
| 中文关键词:羌活胜湿汤 经典名方 网络药理学 指纹图谱 质量标志物 升麻素苷 二氢欧山芹醇当归酸酯 蛇床子素 |
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| Establishment of Fingerprint of Classic Prescription Qianghuo Shengshi Decoction and Predictive Analysis of Functional Substances |
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| Abstract:Objective Based on the method of network pharmacology and the establishment of the fingerprint of the classic prescription Qianghuo Shengshi Decoction (QSD), the quality marker (Q-marker) of QSD was analyzed and predicted.Method The fingerprint of QSD was established by HPLC. The common peaks were assigned and identified, and the active components were screened based on measurability and traceability. The target and pathway were screened by network pharmacology, and the "component-target-pathway" interaction network diagram of QSD was constructed to predict the Q-marker of QSD.Results A total of 20 common peaks were calibrated. Ten chromatographic peaks were identified by comparing with the reference substances, including prim-O-glucosylcimifugin, senkyunolide Ⅰ, osthole, nodakenin, glycyrrhizic acid ammonium salt, ligustilide, ferulic acid, isoimperatorin, 5-O-methylvisammioside and columbianadin, and the similarities were all greater than 0.862. The "component-target-pathway" network of six active components was constructed and analyzed by network pharmacology, the enriched pathways included proteoglycans in cancer, pathways in cancer, chemical carcinogenesis - receptor activation, endocrine resistance and so on. According to the connectivity between components, targets and pathways, it was preliminarily predicted that columbianadin, prim-O-glucosylcimifugin, glycyrrhizic acid ammonium salt, isoimperatorin, osthole and 5-O-methylvisammioside may achieve the functions of expelling wind, eliminating dampness and relieving pain by regulating these signal pathways.Conclusion By establishing the QSD fingerprint to analyze and predict the Q-marker in QSD based on network pharmacology methods, the quality control standards of QSD are further clarified, which is beneficial for improving its quality control level, and also provides a reference for the in-depth study of the mechanism of action of QSD in the later stage. |
| keywords:Qianghuo Shengshi Decoction classic prescription network pharmacology fingerprint Q-marker prim-O-glucosylcimifugin columbianadin osthole |
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