三七须根指纹图谱及三七须根中皂苷成分研究
投稿时间:2024-08-12  修订日期:2024-10-28   点此下载全文
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作者中文名作者英文名单位中文名单位英文名E-Mail
张红燕 zhanghongyan 云南云科特色植物提取实验室有限公司 Yunnan Yunke Characteristic Plant Extraction Laboratory Co., Ltd. 2563321904@qq.com 
杨淳皓 yangchunhao 云南云科特色植物提取实验室有限公司 Yunnan Yunke Characteristic Plant Extraction Laboratory Co., Ltd. yangchunhao@botanee.com 
杨兆祥 yangzhaoxiang 云南云科特色植物提取实验室有限公司 Yunnan Yunke Characteristic Plant Extraction Laboratory Co., Ltd. zhanghongyan@botanee.com 
肖丰坤* xiaofengkun 云南云科特色植物提取实验室有限公司 云南云科特色植物提取实验室有限公司 xiaofengkun@botanee.com 
基金项目:云南特色植物提取实验室自主研究项目(三七须根标准提取物及功效成分开发;基于云南特色植物的舒缓系列产品开发及产业化)
中文摘要:[摘要] 目的:建立三七须根高效液相色谱(HPLC)指纹图谱,对比三七须根和主根中主要皂苷成分的差异,并分析多因素对三七须根中三七皂苷R2(S)和人参皂苷Rh1含量的影响。方法:运用HPLC分析方法绘制样品的指纹图谱,采用中药色谱指纹图谱相似度评价系统2.0版进行相似度评价,确定共有峰;同时检测三七须根和主根中主要皂苷成分的含量。结果:建立了三七须根药材指纹图谱,共确认了11个共有峰,指认了7个成分,其中三七须根中三七皂苷R2(S)和人参皂苷Rh1的含量在总皂苷中的占比显著大于主根。林下种植模式下三七皂苷R2(S)和人参皂苷Rh1的含量在总皂苷中的占比显著高于传统种植模式;不同干燥方式下三七皂苷R2(S)和人参皂苷Rh1含量依次为,鲜品>烘干>阴干>晒干。结论:该研究建立的三七须根指纹图谱稳定可靠,对三七须根中三七皂苷R2(S)和人参皂苷Rh1两个成分进行全面研究,为三七须根的开发利用提供参考。。
中文关键词:三七须根  指纹图谱  三七皂苷R2(S)  人参皂苷Rh1
 
The fingerprint Panax notoginseng fibrous root and its ginsenosides distribution
Abstract:[Abstract]Objective: To establish the high-performance liquid chromatography (HPLC)?fingerprint of?Panax notoginseng?fibrous root,?and analyze the differences in ginsenoside distribution?between the main foot and fibrous root. The article also discussed the?influence of?various factors on the content of?ginsenoside in Panax. notoginseng?fibrous?root. Methods: The HPLC fingerprints were established by the similarity evaluation system of the chromatographic fingerprinting technique of traditional Chinese medicine (TCM) version 2.0, and quantitatived analysis of major saponin components in P. notoginseng?fibrous?roots and main roots. Results: HPLC fingerprints of Panax notoginseng fibrous?root were established with?11 common peaks determination?and seven ginsenosides identification. We highlighted the portions of notoginseng R2(S) and ginsenoside Rh1 in Total Panax Notoginseng saponin (TPNS), which?were significantly higher in the fibrous root?rather?than?the main root. Besides, the geographical position, planting mode, and drying method were analyzed for the attribution to ginsenoside content. The portions of notoginseng R2(S) and ginsenoside Rh1 in TPNS were higher in understory planting mode rather than in?traditional planting mode;?Drying methods influenced the content of notoginseng R2(S) and ginsenoside Rh1 significantly, which were in order of Fresh?>?Oven drying > Shade-drying > Sun-drying.Conclusion: The fingerprint of Panax notoginseng fibrous?root?established in this study is stable and reliable, and the two components of notoginseng R2(S) and ginsenoside Rh1?in Panax notoginseng fibrous?root?are comprehensively studied, which provides a reference for the development and utilization of Panax notoginseng fibrous?root.
keywords:Panax notoginseng fibrous root  HPLC fingerprint  Notoginseng R2(S)  Ginsenoside Rh1
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