| 双标线性校正法联合一测多评法同时测定清热八味胶囊中9个组分的含量 |
| 投稿时间：2024-08-21 修订日期：2024-10-11 点此下载全文
|
| 引用本文： |
| DOI： |
| 摘要点击次数: 0 |
| 全文下载次数: 0 |
|
|
|
| 中文摘要:目的: 建立同时测定蒙成药清热八味胶囊中9个组分(胆红素、羟基红花黄色素A、芦丁、胡黄连苷Ⅱ、胡黄连苷Ⅰ、肉桂酸、槲皮素、紫堇灵、山柰素)的一测多评含量测定方法(QAMS)。方法: 采用高效液相色谱法,安捷伦Zorbax Eclipse Plus C18色谱柱(250mm×4.6mm,5μｍ),以乙腈-0.4％磷酸水溶液为洗脱流动相,进行梯度洗脱,流速1.0ｍL.min-1,检测波长275、370、403,柱温为35℃。以羟基红花黄色素A和胡黄连苷Ⅰ为双标化合物,使用双标线性校正法(LCTRC)法预测保留时间。以胡黄连苷Ⅱ为内参物,建立与其他8个组分的相对校正因子,并计算9个待测组分的含量,实现一测多评。同时与外标法进行比较,以验证QAMS法的准确性和可行性。结果: 9组分在各自试验浓度范围内的线性关系良好(r≥0.9990),平均加样回收率为93.0％～99.8％(n=６),RSD为0.23％～1.53％；建立QAMS法用于测定清热八味胶囊中9个指标性组分的含量,并对6批清热八味胶囊进行测定,其计算值与测定值的差异较小(P＞0.05) 。结论：QAMS法测定清热八味胶囊中9种组分准确且可行,可用于清热八味胶囊的质量控制。 |
| 中文关键词:一测多评法 清热八味胶囊 蒙成药 双标线性校正法 含量测定 |
| |
| Simultaneous determination of 9 components in Qingre Bawei Capsules by LCTRC and QAMS |
|
|
| Abstract:Objective: To establish a quantitative analysis of multi-components by singlemarker (QAMS) for simultaneous determination of bilirubin，hydroxysafflor yellow A，Rutin, picroside-II，picroside-I，cinnamic acid，quercetin，corynoline and kaempferol in Qingre Bawei Capsules.Methods: The HPLC system considered the agilent Zorbax Eclipse Plus C18 column (250mm×4.6mm, 5 μm) with gradient elution of acetonitrile and 0.4% phosphoric acid as the mobile phase at a flow rate of 1.0ｍL?min-1, a detection wavelength of 275,370 and 403nm, and a column temperature of 35℃. Both hydroxysafflor yellow A and picroside-I were taken as the two reference substances to forecast the retention time of the other three components by linear calibration with two reference substances （LCTRC）. picroside-II was selected as the international reference substance, the relative correction factors between picroside-II and the other 8 onstitutions were established, and the content of these 9 constitutions in samples were calculated to realize QAMS. At the same time, compare with the external standard method to verify the accuracy and feasibility of the QAMS method.Results: All 9 components exhibited good linear relationships are within the respective concentration ranges (r≥0.9990) . The average recoveries (n=6) ranged from 93.0% to 99.8% , with relative standard deviations (RSDS) of 0.23% to 1.53% . The established QAMS was used to determine the 9 Index components in Qingre Bawei Capsules,and totally 6 batches of Qingre Bawei Capsules were determined．There was no significant difference in the calculated values and the determined ones (P＞0.05).Conclusion：The QAMS method is feasible and accurate for the determination of the 4 chemical compositions, and which can be used for quality control of Qingre Bawei Capsules. |
| keywords:Quantitative analysis of multi-components by single marker Qingre Bawei Capsules Mongolian medicine Linear calibration with two reference substances Content determination |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
