| 顺-3-己烯醛在黄芪根培养体系中的应用 |
| 投稿时间：2024-08-30 修订日期：2024-12-17 点此下载全文
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| 基金项目:]山西省自然科学基金面上项目(编号：202103021224017)；山西省黄芪资源产业化及产业国际化协同创新中心项目(HQXTCXZX2016-002,HQXTCXZX2016-003,HQXTCXZX2016-016)。 |
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| 中文摘要:目的：优化顺-3-己烯醛处理条件下的黄芪不定根液体培养体系，建立黄芪皂苷分析用培养液样品制备方法，明确对黄芪皂苷累积的影响及释放特征。方法：考察50 mL三角瓶中培养液体积（20 mL、30 mL）、接种量（培养液体积的5.0%、7.5%、10.0%）、顺-3-己烯醛添加量（12.5 μmol·L-1、50.0 μmol·L-1、200.0 μmol·L-1）对摇床培养4周根生长及黄芪皂苷含量的影响，优化培养体系；利用D101大孔树脂进行培养液除杂、富集及样品制备；采用最佳方案顺-3-己烯醛处理根培养物3 d，动态取样并测定根培养物和培养液中黄芪皂苷及环黄芪醇含量。结果：培养液体积为20 mL、接种量为1.0 g的体系，添加12.5 μmol·L-1顺-3-己烯醛促进根生长和黄芪皂苷累积效应最佳；30 mL为一柱体积的D101大孔树脂可完全吸附20~200 mL培养液中的黄芪皂苷，且黄芪皂苷Ⅳ、异黄芪皂苷Ⅰ、异黄芪皂苷Ⅱ、异黄芪皂苷Ⅳ主要存在于培养液中；12.5 μmol·L-1顺-3-己烯醛诱导处理4~8 h，促进黄芪皂苷Ⅰ、环黄芪苷Ⅱ效应最佳。结论：20 mL的黄芪不定根液体培养体系中，添加12.5 μmol·L-1顺-3-己烯醛，有助于根培养物的生长和黄芪皂苷的积累。 |
| 中文关键词:蒙古黄芪 不定根培养 顺-3-己烯醛 C6挥发物 黄芪皂苷 |
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| Research on the Promoting Effects of Cis-3-hexenal on the Biosynthesis and Accumulation of Triterpenoid Saponins in Astragalus membranaceus var. mongholicus Root Cultures |
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| Abstract:Objective: To optimize the root culture system of Astragalus membranaceus var. mongholicus (Bunge) P.K. Hsiao. in the presence of cis-3-hexenal, to establish preparation method of the liquid samples for measurements of astragalosides, and to clarify the impact on the accumulation and release of astragalosides into the medium. Methods: The effects of the medium volume (20 mL, 30 mL), the inoculum size (5.0%, 7.5%, 10.0% of the medium volume), and the concentration of cis-3-hexenal (12.5 μmol·L?1, 50.0 μmol·L?1, 200.0 μmol·L?1) in 50 mL Erlenmeyer flasks on the root growth and the content of astragalosides were examined to optimize the culture system after 4 weeks’ incubation. D101 macroporous resin was used for impurity removal, enrichment, and preparation of the liquid samples. Using the optimized system, a 3-days’ incubation was performed, and the contents of astragalosides and cycloastragenol in the root cultures and in the liquids were dynamically determined. Results: When the liquid volume was 20 mL and the inoculum size was 1.0 g, 12.5 μmol·L?1 cis-3-hexenal promoted the root growth and the accumulation of astragalosides to the highest level. A column volume of 30 mL of D101 macroporous resincould completely adsorb astragalosides in 20 - 200 mL of the liquid medium, and astragaloside Ⅳ, isoastragaloside Ⅰ, isoastragaloside Ⅱ, and isoastragaloside Ⅳ were mainly released into the medium. And the treatment of 12.5 μmol·L?1 cis-3-hexenal for 4 - 8 h showed the most effective promotion of astragaloside Ⅰ and cycloastragenoside Ⅱ. Conclusion: Adding 12.5 μmol·L?1 cis-3-hexenal to the root culture system of A. mongholicus was beneficial to the growth of the root cultures and to the accumulation of astragalosides. |
| keywords:Astragalus mongholicus, adventitious root culture, cis-3-hexenal, six-carbon volatiles, astragalosidee |
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