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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
王琳	WANG Lin	山东中医药大学药学院，山东济南 250355 山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China
魏霞	WEI Xia	山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China
焦阳	JIAO Yang	山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China
汪冰	WANG Bing	山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China
解盈盈	XIE Ying-ying	山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China
尹雪	YIN Xue	山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China
石丽	SHI Li	山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China
陈娟	CHEN Juan	山东中医药大学药学院，山东济南 250355 山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China
孙仟	SUN Qian	山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China
刘丽	LIU Li	山东中医药大学药学院，山东济南 250355 山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China
张加余*	ZHANG Jia-yu	山东中医药大学药学院，山东济南 250355 滨州医学院中医学院，山东烟台 264003	School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China School of Traditional Chinese Medicine, Binzhou Medical University, Yantai 264003, China
林永强*	LIN Yong-qiang	山东省食品药品检验研究院国家药品监督管理局胶类产品质量评价重点实验室/山东省中药标准创新与质量评价工程实验室/中药配方颗粒共性技术山东省工程研究中心，山东济南 250101	Shandong Institute for Food and Drug Control/NMPA Key Laboratory for Quality Evaluation of Gelatin Products/Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM/Shandong Engineering Research Center for Generic Technologies of Traditional Chinese Medicine Formula Granules, Jinan 250101, China

基金项目:国家重点研发计划项目（2023YFC3504102）；山东省重点研发计划（重大科技创新工程）项目（2021CXGC010511）；泉城产业领军人才支持计划创新团队项目

中文摘要:目的采用性状鉴别和聚合酶链式反应（PCR）技术对我国主要的海马养殖品膨腹海马Hippocampus abdominalis Leeson进行研究，为该品种鉴别技术开发提供数据支持。方法通过查阅文献资料并结合样品统计观察，总结膨腹海马的性状鉴别特征；根据膨腹海马及其他海马的细胞色素C氧化酶亚基Ⅰ（COⅠ）基因序列，筛选出膨腹海马的特异性单核苷酸多态性（SNP）位点，设计专属膨腹海马的引物PFHM-Primer-F和PFHM-Primer-R。通过优化PCR体系反应条件，考察最佳的PCR反应体系。结果膨腹海马的核心性状特征主要包括有较多的体环、尾环和背鳍条数，背鳍覆盖4个体环+1个尾环；眼周和鳃盖部具有明显的大而黑的斑点等。基于COⅠ序列展开的PCR鉴别方法研究表明，当退火温度为65 ℃，循环30次时，膨腹海马样品在100~250 bp呈现单一明亮条带，而其他品种均无此条带。结论建立的性状鉴别和特异性PCR鉴别方法可快速鉴别膨腹海马，为我国海马新资源的鉴别和后续开发提供参考。

中文关键词:膨腹海马细胞色素C氧化酶亚基Ⅰ 特异性聚合酶链式反应性状鉴别

Identification of Hippocampus abdominalis Based on the Combination of the Morphological Characteristics and Specific PCR

Abstract:Objective Morphological characteristics and polymerase chain reaction (PCR) techniques were used to investigate Hippocampus abdominalis Leeson, the main cultivated species of seahorse in China, to provide data support for the development of identification techniques for this species.Methods Through literature review and statistical observation of samples, morphological identification characteristics of H. abdominalis were summarized. According to the cytochrome C oxidase subunit Ⅰ (COⅠ) gene sequences of the H. abdominalis and other seahorses, specific single nucleotide polymorphism (SNP) sites were screened, and the specific primers PFHM-Primer-F and PFHM-Primer-R were designed. By optimizing the reaction conditions of the PCR system, the optimal PCR reaction system was determined.Results The core morphological characteristics of the H. abdominalis mainly include more body rings, tail rings, and dorsal fins, with the dorsal fin covering 4 body rings +1 tail ring, and noticeable large and black spots around periocular and operculum areas, etc. The PCR identification method based on the COⅠ sequence showed that when the annealing temperature was 65 ℃ and cycled 30 times, H. abdominalis samples displayed a single bright band in the 100-250 bp, while other species showed no such band.Conclusion The morphological identification and specific PCR identification methods established in this study can quickly identify H. abdominalis, providing a reference for the identification and subsequent development of new seahorse resources in China.

keywords:Hippocampus abdominalis Leeson COⅠ specific PCR character identification

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