| 基于LC-MS联合多元统计分析的百合鲜品及其干燥品差异性分析 |
| 投稿时间：2023-12-25 点此下载全文
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| 引用本文：王碧莹,张欣亚,陈随清,付钰.基于LC-MS联合多元统计分析的百合鲜品及其干燥品差异性分析[J].中国现代中药,2024,26(9):1511-1519 |
| DOI：10.13313/j.issn.1673-4890.20231225005 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 王碧莹 |
WANG Bi-ying |
河南中医药大学 药学院，河南 郑州 450046 |
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China |
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| 张欣亚 |
ZHANG Xin-ya |
河南中医药大学 药学院，河南 郑州 450046 |
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China |
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| 陈随清* |
CHEN Sui-qing |
河南中医药大学 药学院，河南 郑州 450046
河南中医药大学 呼吸疾病中医药防治省部共建协同创新中心，河南 郑州 450046 |
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China
Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province & Education Ministry of P.R.China, Henan University of Chinese Medicine, Zhengzhou 450046, China |
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| 付钰* |
FU Yu |
河南中医药大学 药学院，河南 郑州 450046 |
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China |
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| 基金项目:河南省中医药科学研究专项课题重大专项（2022ZYZD18）；河南省高等学校重点科研项目（24A360004） |
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| 中文摘要:目的 基于超高效液相色谱-四级杆飞行时间质谱法（UPLC Q-TOF MS）对比不同种百合（卷丹及百合）的新鲜品和干燥品（冷冻干燥及烘干）成分的差异。方法 采用UPLC Q-TOF MS技术，对不同种百合的新鲜品和干燥品进行分析，进一步通过主成分分析（PCA）、正交偏最小二乘法-判别分析（OPLS-DA）结合单因素分析筛选百合新鲜样品及烘干样品的差异化合物。结果 UPLC Q-TOF MS结果显示，百合新鲜样品和干燥品主要成分相似。PCA结果显示，百合新鲜品与冻干品聚为一类，而两者与烘干品有明显差异，说明百合冻干样品和新鲜样品整体化学成分相近，而烘干样品经过烫片和烘干处理后化学成分发生了一定变化，故与前两者相异。OPLS-DA结果显示，卷丹新鲜品与烘干品存在387个差异成分，百合新鲜品与烘干品存在335个差异成分，经数据库比对，初步鉴定出8个成分。结论 相较于烘干百合，鲜百合与冷冻干燥百合成分更为相近，为百合药材临床应用与现代中药制剂开发提供依据。 |
| 中文关键词:百合 新鲜药材 干燥药材 超高效液相色谱-四级杆飞行时间质谱法 |
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| Differential Analysis of Fresh and Dried Lilii Bulbus Using LC-MS Combined with Multivariate Statistical Analysis |
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| Abstract:Objective To compare the differences in components between fresh and dried (freeze-dried and oven-dried) samples of different species of Lilii Bulbus (Lilium lancifolium Thunb. and L. brownii F.E.Brown var. viridulum Baker) based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC Q-TOF MS).Methods UPLC Q-TOF MS technology was employed to analyze fresh and dried samples of different Lilii Bulbus species. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) combined with single-factor analysis were employed to screen for differential compounds in fresh and oven-dried Lilii Bulbus samples.Results The UPLC Q-TOF MS showed that the main components of fresh and dried Lilii Bulbus samples were similar. PCA results showed that fresh and freeze-dried Lilii Bulbus samples clustered into one group, while both differed significantly from oven-dried samples. This suggests that the overall chemical compounds of freeze-dried Lilii Bulbus samples are similar to fresh samples. In contrast, the chemical composition of oven-dried samples changed after blanching and drying, making them different from the former two. OPLS-DA results showed 387 differential components between fresh and oven-dried Lilium lancifolium Thunb. samples and 335 differential components between fresh and oven-dried Lilium Bulbus samples. 8 components were preliminarily identified through database comparison.Conclusion Compared to oven-dried Lilium Bulbus, the components of fresh and freeze-dried Lilii Bulbus are more similar, providing a basis for the clinical application and modern Chinese medicine formulation development of Lilii Bulbus. |
| keywords:Lilii Bulbus fresh medicinal materials dried medicinal materials UPLC Q-TOF MS |
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