| 基于PLS-DA的盘龙七及其易混品大盘龙七差异性成分分析 |
| 投稿时间：2024-01-31 点此下载全文
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| 引用本文：何姬,刘妍如,段金廒,唐志书,宋忠兴,赵艳婷,游雪莲,张德柱,杨国伟,张丽,权伟.基于PLS-DA的盘龙七及其易混品大盘龙七差异性成分分析[J].中国现代中药,2024,26(9):1520-1527 |
| DOI：10.13313/j.issn.1673-4890.20240131004 |
| 摘要点击次数: 1438 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 何姬 |
HE Ji |
陕西中医药大学/陕西中药资源产业化省部共建协同创新中心，陕西 咸阳 712083 |
Shaanxi University of Chinese Medicine/Shaanxi Chinese Medicine Resources Industrialization Collaborative Innovation Center, Xianyang 712083, China |
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| 刘妍如* |
LIU Yan-ru |
陕西中医药大学/陕西中药资源产业化省部共建协同创新中心，陕西 咸阳 712083 |
Shaanxi University of Chinese Medicine/Shaanxi Chinese Medicine Resources Industrialization Collaborative Innovation Center, Xianyang 712083, China |
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| 段金廒* |
DUAN Jin-ao |
南京中医药大学/江苏省中药资源产业化过程协同创新中心/江苏省方剂高技术研究重点实验室， 江苏 南京 210023 |
Jiangsu Key Laboratory for High Technology Research of TCM Formulae Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, China |
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| 唐志书 |
TANG Zhi-shu |
陕西中医药大学/陕西中药资源产业化省部共建协同创新中心，陕西 咸阳 712083
北京中医药大学 中药学院，北京 100700 |
Shaanxi University of Chinese Medicine/Shaanxi Chinese Medicine Resources Industrialization Collaborative Innovation Center, Xianyang 712083, China
School of Chinese Materia Medica.Beijing University of Chinese Medicine, Beijing 100700, China |
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| 宋忠兴 |
SONG Zhong-xing |
陕西中医药大学/陕西中药资源产业化省部共建协同创新中心，陕西 咸阳 712083 |
Shaanxi University of Chinese Medicine/Shaanxi Chinese Medicine Resources Industrialization Collaborative Innovation Center, Xianyang 712083, China |
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| 赵艳婷 |
ZHAO Yan-ting |
陕西中医药大学/陕西中药资源产业化省部共建协同创新中心，陕西 咸阳 712083 |
Shaanxi University of Chinese Medicine/Shaanxi Chinese Medicine Resources Industrialization Collaborative Innovation Center, Xianyang 712083, China |
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| 游雪莲 |
YOU Xue-lian |
陕西中医药大学/陕西中药资源产业化省部共建协同创新中心，陕西 咸阳 712083 |
Shaanxi University of Chinese Medicine/Shaanxi Chinese Medicine Resources Industrialization Collaborative Innovation Center, Xianyang 712083, China |
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| 张德柱 |
ZHANG De-zhu |
陕西盘龙药业集团股份有限公司，陕西 商洛 711400 |
Shaanxi Panlong Pharmaceutical Group Co., Ltd., Shangluo 711400, China |
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| 杨国伟 |
YANG Guo-wei |
山西省检验检测中心/山西省标准计量技术研究院，山西 太原 030002 |
Shanxi Inspection and Testing Center (Shanxi Institute of Standards and Metrology), Taiyuan 030002, China |
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| 张丽 |
ZHANG Li |
陕西中医药大学 附属医院 药剂科，陕西 咸阳 712000 |
Department of Pharmacy, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China |
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| 权伟 |
QUAN Wei |
陕西中医药大学 附属医院 药剂科，陕西 咸阳 712000 |
Department of Pharmacy, Affiliated Hospital of Shaanxi University of Chinese Medicine, Xianyang 712000, China |
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| 基金项目:陕西省重点研发计划项目（2020ZDLSF05-08）；陕西省高层次人才特殊支持计划项目（陕组通字〔2018〕33号） |
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| 中文摘要:目的 确定盘龙七及其同名异物药材大盘龙七的差异性成分并建立其含量测定方法。方法 采用偏最小二乘法-判别分析（PLS-DA）结合超高效液相色谱-四极杆-飞行时间质谱法（UPLC-Q-TOF-MS）分析盘龙七和大盘龙七差异性成分，并建立差异性成分定性、定量分析方法。结果 PLS-DA结果显示以t检验（P<0.05）和变量重要性投影（VIP）值（VIP>1.0）为标准，筛选出盘龙七和大盘龙七差异性成分共296个。对排名靠前的差异性成分进行表征分析，进一步筛选出符合碎片裂解规律及文献记载的差异性成分共8个，其中绿原酸、没食子酸、岩白菜素3个成分含量差异最显著。结论 该方法能有效分析盘龙七和大盘龙七的差异性，为盘龙七及大盘龙七的质量评价及定性、定量分析提供参考。 |
| 中文关键词:盘龙七 大盘龙七 偏最小二乘法-判别分析 超高效液相色谱-四极杆-飞行时间质谱法 绿原酸 没食子酸 岩白菜素 |
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| Analysis of Differential Components of Polygonum milletii and the Confused Product Bergenia scopulosa Based on PLS-DA |
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| Abstract:Objective To identify the differential components and establish a methodology for content determination of Polygonum milletii and its homonym herb Bergenia scopulosa.Methods Partial least squares discriminant analysis (PLS-DA) combined with ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry (UPLC-Q-TOF-MS) was used to analyze the differential components of P. milletii and B. scopulosa and to establish a methodology for their qualitative and quantitative determination.Results The PLS-DA results showed that 296 differential components of P. milletii and B. scopulosa were identified using the t-test (P<0.05) and VIP value (VIP>1.0) as criteria. The top-ranked differential components were characterized and analyzed, resulting in the identification of eight differential components that conformed to fragmentation patterns and literature. Among these, the content differences in three components, i.e., chlorogenic acid, gallic acid, and bergenin, were the most significant.Conclusion This method can effectively analyze the differences between P. milletii and B. scopulosa and provide a reference for the quality evaluation and qualitative and quantitative analysis of P. milletii and B. scopulosa. |
| keywords:Polygonum Milletii Rhizoma Bergeniae Scopulosae Rhizoma PLS-DA UPLC-Q-TOF-MS chlorogenic acid gallic acid bergenin |
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