| 基于HPLC测定4种藏茵陈原植物中10个有效成分含量 |
| 投稿时间：2024-01-02 点此下载全文
|
| 引用本文：张楠,许苗苗,杜赟赟,刘凤凤,陈洪卫,谢志民.基于HPLC测定4种藏茵陈原植物中10个有效成分含量[J].中国现代中药,2024,26(9):1528-1534 |
| DOI：10.13313/j.issn.1673-4890.20240102002 |
| 摘要点击次数: 1511 |
| 全文下载次数: 0 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 张楠* |
ZHANG Nan |
西安市食品药品检验所，陕西 西安 710054 |
Xi'an Institute for Food and Drug Control, Xi'an 710054, China |
|
| 许苗苗 |
XU Miao-miao |
西安市食品药品检验所，陕西 西安 710054 |
Xi'an Institute for Food and Drug Control, Xi'an 710054, China |
|
| 杜赟赟 |
DU Yun-yun |
西安市食品药品检验所，陕西 西安 710054 |
Xi'an Institute for Food and Drug Control, Xi'an 710054, China |
|
| 刘凤凤 |
LIU Feng-feng |
西安远大德天药业股份有限公司，陕西 西安 710119 |
Xi'an Grand Deten Pharmaceutical Co., Ltd., Xi'an 710119, China |
|
| 陈洪卫 |
CHEN Hong-wei |
西安远大德天药业股份有限公司，陕西 西安 710119 |
Xi'an Grand Deten Pharmaceutical Co., Ltd., Xi'an 710119, China |
|
| 谢志民 |
XIE Zhi-min |
西安市食品药品检验所，陕西 西安 710054 |
Xi'an Institute for Food and Drug Control, Xi'an 710054, China |
|
|
|
| 中文摘要:目的 建立高效液相色谱法（HPLC）测定藏茵陈中獐牙菜苦苷、芒果苷、龙胆苦苷、当药苷、异荭草苷、异牡荆苷、当药黄素、当药醇苷、甲基当药宁、齐墩果酸含量的方法。方法 采用Welch Ultimate XB-C18色谱柱，以乙腈-0.1%磷酸水溶液为流动相，梯度洗脱，体积流量为1.0 mL·min–1，獐牙菜苦苷、芒果苷、龙胆苦苷、当药苷测定波长为240 nm，异荭草苷、异牡荆苷、当药黄素测定波长为270 nm，当药醇苷、甲基当药宁测定波长为250 nm，齐墩果酸检测波长为205 nm，进样量为10 μL。结果 该条件下指标成分进样质量分别为獐牙菜苦苷126.7~16 040.0 ng（r=0.999 8）、芒果苷6.3~801.6 ng（r=0.999 6）、龙胆苦苷6.4~814.4 ng（r=0.999 7）、当药苷15.9~2 016.0 ng（r=0.999 9）、异荭草苷7.1~900.0 ng（r=0.999 5）、异牡荆苷5.5~699.2 ng（r=0.999 7）、当药黄素5.4~681.6 ng（r=0.999 8）、当药醇苷5.4~685.6 ng（r=0.999 6）、甲基当药宁19.3~2 438.0 ng（r=0.999 7）、齐墩果酸13.6~1 716.0 ng（r=0.999 8）时与峰面积具有较好的线性关系；方法学考察结果显示精密度（RSD≤0.95%）和重复性（RSD≤1.86%）良好，供试品溶液在室温条件下24 h内稳定，RSD≤1.85%；平均加样回收率和相应的RSD分别为99.56%（0.58%）、100.37%（1.23%）、98.63%（0.59%）、99.11%（1.02%）、98.64%（0.57%）、102.26%（0.88%）、100.37%（0.59%）、97.05%（1.36%）、96.01%（0.45%）、101.55%（0.49%）。18批藏茵陈原植物中獐牙菜苦苷、芒果苷、龙胆苦苷、当药苷、异荭草苷、异牡荆苷、当药黄素、当药醇苷、甲基当药宁、齐墩果酸的质量分数差异较大，分别为0.111%~6.592%、0.195%~1.959%、0.046%~4.139%、0.058%~0.662%、0.090%~1.421%、0.082%~0.283%、0.061%~0.384%、0.022%~1.589%、0.106%~1.858%、0.160%~0.657%。结论 建立的HPLC同时测定藏茵陈中10个有效成分含量，方法简便、快速、准确，可为藏茵陈质量控制及资源有效开发提供参考。 |
| 中文关键词:高效液相色谱法 藏茵陈 有效成分 含量测定 |
| |
| Determination of Contentof Ten Active Componentsin Four Plant Species Producing Zangyinchen by HPLC |
|
|
| Abstract:Objective To establish a method based on high performance liquid chromatography (HPLC) for simultaneous determination of 10 active components (swertiamarin, mangiferin, gentiopicroside, sweroside, isoorientin, isovitexin, swertisin, swertianolin,swertiaperennin,and oleanolic acid) in Zangyinchen.Methods The chromatographic separation was achieved bygradient elutionthrougha Welch Ultimate XB-C18 column with acetonitrile-0.1% phosphoric acid solution as the mobile phaseat the flow rate of 1.0 mL·min–1.The detection wavelength was set at 240 nm for swertiamarin, mangiferin, gentiopicroside, and sweroside, 270 nm for isoorientin, isovitexin, and swertisin,250 nm for swertianolin andswertiaperennin, and 205 nm for oleanolicacid.The volume of sample injection was 10 μL.Results The ten active components were well separated. Swertiamarin, mangiferin,gentiopicroside,sweroside,isoorientin,isovitexin,swertisin,swertianolin, swertiaperennin, andoleanolic acidshowed good linearity with the peak areaswithin the ranges of 126.7-16 040.0 ng (r=0.999 8), 6.3-801.6 ng (r=0.999 6), 6.4-814.4 ng (r=0.999 7), 15.9-2 016.0 ng (r=0.999 9), 7.1-900.0 ng (r=0.999 5), 5.5-699.2 ng (r=0.999 7), 5.4-681.6 ng (r=0.999 8), 5.4-685.6 ng (r=0.999 6), 19.3-2 438.0 ng (r=0.999 7), and 13.6-1 716.0 ng (r=0.999 8), respectively. The established method demonstrated good precision and repeatability, with the relative standard deviations (RSDs)≤0.95% and ≤1.86%, respectively. The test solutions were stable in 24 h, withthe RSDs no more than 1.85%. The average spiked recovery and corresponding RSDs were 99.56% (0.58%), 100.37% (1.23%), 98.63% (0.59%), 99.11% (1.02%), 98.64% (0.57%), 102.26% (0.88%), 100.37% (0.59%), 97.05% (1.36%),96.01% (0.45%), and 101.55% (0.49%), respectively. The content of swertiamarin, mangiferin, gentiopicroside, sweroside, isoorientin, isovitexin, swertisin, swertianolin, swertiaperennin, andoleanolic acid in 18 batches of Zangyinchen samples was 0.111%- 6.592%, 0.195%-1.959%, 0.046%-4.139%, 0.058%-0.662%, 0.090%-1.421%, 0.082%-0.283%,0.061%-0.384%, 0.022%- 1.589%, 0.106%-1.858%, and 0.160%-0.657%, respectively.Conclusion An HPLC method was established for the simultaneous determination of ten components in Zangyinchen, being simple, quick, and accurate. The findings lay a foundation for the quality control of Zangyinchen and promote the comprehensive applicationof the plants. |
| keywords:high performance liquid chromatography (HPLC) Zangyinchen active component content determination |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
