| 防风多糖SP-3抗肿瘤活性及其机制研究 |
| 投稿时间：2024-02-02 点此下载全文
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| 引用本文：王凯赫,秦铭,王石峰,刘湉,母磊鑫,柯彩依,徐广,马群.防风多糖SP-3抗肿瘤活性及其机制研究[J].中国现代中药,2024,26(9):1535-1543 |
| DOI：10.13313/j.issn.1673-4890.20240202001 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 王凯赫 |
WANG Kai-he |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 秦铭 |
QIN Ming |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 王石峰 |
WANG Shi-feng |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 刘湉 |
LIU Tian |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 母磊鑫 |
MU Lei-xin |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 柯彩依 |
KE Cai-yi |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 徐广 |
XU Guang |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 马群* |
MA Qun |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 基金项目:国家中医药管理局高水平中医药重点学科建设项目（zyyzdxk-2023265） |
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| 中文摘要:目的 研究防风多糖SP-3的抗肿瘤活性及作用机制。方法 通过噻唑蓝（MTT）法、流式细胞术检测防风多糖SP-3对癌细胞增殖、细胞周期及凋亡的影响，采用秀丽隐杆线虫（以下简称线虫）模型研究防风多糖SP-3抗肿瘤活性机制。结果 防风多糖SP-3可以显著抑制胃癌AGS细胞、肝癌HepG2细胞的增殖，促进胃癌AGS细胞的凋亡并影响其细胞周期，且呈剂量依赖性；防风多糖SP-3对大鼠肉瘤（Ras）基因突变的线虫多阴门表型具有显著的抑制作用。结论 防风多糖SP-3具有良好的抗肿瘤效果，其作用机制可能与Ras/丝裂原活化蛋白激酶（MAPK）通路有关。 |
| 中文关键词:防风多糖SP-3 胃癌AGS细胞 秀丽隐杆线虫 大鼠肉瘤/丝裂原活化蛋白激酶 |
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| Antitumor Activity and Mechanism of Saposhnikovia divaricata Polysaccharide SP-3 |
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| Abstract:Objective To study the antitumor activity and mechanism of Saposhnikovia divaricata polysaccharide SP-3.Methods The effects of SP-3 on cancer cell proliferation, cell cycle, and apoptosis were detected using the MTT assay and flow cytometry. The antitumor activity mechanism of SP-3 was studied using the Caenorhabditis elegans model.Results SP-3 significantly inhibited the proliferation of gastric cancer AGS cells and hepatoma HepG2 cells, promoted apoptosis in gastric cancer AGS cells, and affected their cell cycle in a dose-dependent manner. Additionally, SP-3 exhibited a notable inhibitory effect on the multivulva phenotype in Ras gene mutant C. elegans.Conclusion SP-3 has a potent antitumor effect, and its mechanism may be related to the Ras/mitogen-activated protein kinase (MAPK) pathway. |
| keywords:Saposhnikovia divaricata polysaccharide SP-3 gastric cancer AGS cells Caenorhabditis elegans Ras/MAPK |
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