| Abstract:Objective: To study the antioxidant capacity of the water extract from the root of Spuriopimpinella brachycarpa and its effect on the life of Caenorhabditis elegans, and to explore its medicinal potential. Methods: The chemical components in the water extract from the root of Spuriopimpinella brachycarpa were identified via UHPLC-Q-Exactive Orbitrap-MS technology. In this study, wild-type and transgenic SOD-3::GFP Caenorhabditis elegans were used to evaluate the antioxidant effects of the water extract from the roots of Spuriopimpinella brachycarpa. An oxidative stress model was induced by 100 mM paraquat, the extracts with different concentrations were treated in L1 stage elegans, and their effects on the antioxidant enzyme activity, reactive oxygen species (ROS) level and life of elegans were evaluated. Results: As revealed by the chemical analysis, the water extract from the root of Spuriopimpinella brachycarpa contained such phenolic acids as chlorogenic acid, caffeic acid and quinic acid, which exhibited significant antioxidant activity. The extract significantly reduced the production of reactive oxygen species (ROS) in the nematodes, restoring them to normal levels, and rescued the decline in SOD-3::GFP expression caused by oxidative stress, thereby maintaining the activity of superoxide dismutase (SOD).The water extract of Spuriopimpinella brachycarpa can also delay the death of elegans induced by paraquat, showing good antioxidant effects and life-prolonging effect. Conclusion: The water extract from the root of Spuriopimpinella brachycarpa can enhance the antioxidant defense system of elegans, reduce the oxidative stress and further prolong their life. This provides a scientific basis for its application as a natural antioxidant, and lays a foundation for the development of plant drugs and their aging research. In the future, it is necessary to further probe into the separation, purification and antioxidant mechanism of its active components, and assess its effect in other model organisms and its clinical application potential. |
