| 基于秀丽隐杆线虫模式生物探讨海风藤多糖抗AD活性 |
| 投稿时间：2024-03-20 点此下载全文
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| 引用本文：温涵,厉昌旭,刘嘉悦,邵梦婷,王艳艳,赵宏.基于秀丽隐杆线虫模式生物探讨海风藤多糖抗AD活性[J].中国现代中药,2024,26(9):1544-1550 |
| DOI：10.13313/j.issn.1673-4890.20240320002 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 温涵 |
WEN Han |
佳木斯大学 药学院，黑龙江 佳木斯 154007 |
School of Pharmacy, Jiamusi University, Jiamusi 154007, China |
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| 厉昌旭 |
LI Chang-xu |
佳木斯大学 药学院，黑龙江 佳木斯 154007 |
School of Pharmacy, Jiamusi University, Jiamusi 154007, China |
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| 刘嘉悦 |
LIU Jia-yue |
佳木斯大学 药学院，黑龙江 佳木斯 154007 |
School of Pharmacy, Jiamusi University, Jiamusi 154007, China |
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| 邵梦婷 |
SHAO Meng-ting |
佳木斯大学 药学院，黑龙江 佳木斯 154007 |
School of Pharmacy, Jiamusi University, Jiamusi 154007, China |
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| 王艳艳 |
WANG Yan-yan |
佳木斯大学 药学院，黑龙江 佳木斯 154007 |
School of Pharmacy, Jiamusi University, Jiamusi 154007, China |
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| 赵宏* |
ZHAO Hong |
佳木斯大学 药学院，黑龙江 佳木斯 154007 |
School of Pharmacy, Jiamusi University, Jiamusi 154007, China |
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| 基金项目:黑龙江省教育厅科技创新团队建设计划项目（2021-KYYEF-0638）；黑龙江省自然科学基金联合项目（LH2022H093）；黑龙江省博士后科研启动金资助项目（LBH-Q20185）；黑龙江省自然科学基金重点项目（ZD2022H006）；黑龙江省北药与功能食品特色学科建设项目（HLJTSXK-2022-03）；佳木斯大学优秀学科团队项目（JDXKTD-2019005） |
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| 中文摘要:目的 探究海风藤分级多糖（KPSP）的抗阿尔茨海默病（AD）活性。方法 采用分级醇沉制备3种醇沉体积分数（40%、60%、80%）的KPSP（KPSP1~KPSP3），通过苯酚-硫酸法测定KPSP的糖含量；以CL4176秀丽隐杆线虫（以下简称线虫）为模型生物，观察KPSP对线虫寿命、瘫痪情况、应激能力及体内活性氧（ROS）自由基、超氧阴离子自由基和β淀粉样蛋白（Aβ）含量的影响。结果 KPSP1~KPSP3中糖质量分数分别为（57.45±0.27）%、（65.32±1.26）%、（70.26±3.53）%，结果显示最佳质量浓度下的KPSP1~KPSP3分别使线虫寿命延长了38.35%、42.02%和39.13%，在一定程度上可以减缓线虫的瘫痪速率，减少线虫体内ROS、超氧阴离子自由基和Aβ含量。结论 KPSP具有较好的抗氧化活性和抗AD活性。 |
| 中文关键词:海风藤 多糖 阿尔茨海默病 抗氧化 秀丽隐杆线虫 |
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| Investigating Anti-Alzheimer's Disease Activity of Kadsura Pepper Stem Polysaccharide Based on Model Organism of Caenorhabditis Elegans |
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| Abstract:Objective To explore the anti-Alzheimer's disease (AD) activity of fractionated polysaccharides from Kadsura Pepper Stem (KPSP).Methods KPSP (KPSP1-KPSP3) was prepared with three ethanol precipitation concentrations (40%, 60%, and 80%) using graded ethanol precipitation. The sugar content of KPSP was determined using the phenol-sulfuric acid method. Caenorhabditis elegans CL4176 (hereafter referred to as nematodes) was used as a model organism to observe the effects of KPSP on nematode lifespan, paralysis, stress capacity, and contents of reactive oxygen species (ROS), superoxide anion radical and β-amyloid protein (Aβ) in nematodes.Results The sugar mass fractions in KPSP1, KPSP2 and KPSP3 were (57.45±0.27)%, (65.32±1.26)%, and (70.26±3.53)%, respectively. The results showed that KPSP at the optimal concentration prolonged the nematode lifespan by 38.35%, 42.02%, and 39.13%, respectively. To a certain extent, it slowed the paralysis rate of the nematodes and reduced the contents of ROS, superoxide anion radical, and Aβ in the nematodes.Conclusion KPSP exhibits good antioxidant activity and anti-Alzheimer's disease activity. |
| keywords:Kadsura Pepper Stem polysaccharide Alzheimer's disease antioxidant Caenorhabditis elegans |
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