| Abstract:Objective: This study aims to compare the components and mechanisms of G. jasminoides Ellis and G. jasminoides var. Radicans Makino through network pharmacology to comprehensively explore the medicinal potential of Shuizhizi. Methods: Using network pharmacology, the main active components of G. jasminoides Ellis and G. jasminoides var. Radicans Makino were screened via TCMSP and SwissADME, with target prediction through STRING and pathway analysis by GO and KEGG to explore potential mechanisms in treating jaundice and hepatitis. Molecular docking compared the binding affinities of key components with related targets. Additionally, an LPS-induced RAW 264.7 cell inflammation model was used to test different concentrations of geniposide, genipin, crocin, crocin II, and crocetin. Cell viability was assessed by CCK-8, NO production by Griess, and TNF-α, IL-6, and IL-1β levels by ELISA to evaluate anti-inflammatory effects.Results: G. jasminoides Ellis and G. jasminoides var. Radicans Makino exhibited common targets for jaundice and hepatitis, with GO and KEGG analyses revealing high similarities and associations with inflammation-related pathways. Toxicity prediction and molecular docking identified five shared compounds—geniposide, genipin, crocin, crocin II, and crocetin—that showed significant proliferative effects on LPS-induced RAW 264.7 cells and notable inhibition of the inflammatory factors NO, TNF-α, IL-6, and IL-1β. Conclusion: Comparative analysis through network pharmacology revealed that G. jasminoides Ellis and G. jasminoides var. Radicans Makino share similar components and mechanisms in treating jaundice and hepatitis. Among them, geniposide, genipin, crocin, crocin II, and crocetin demonstrated significant therapeutic potential. These findings provide new perspectives and references for the development of G. jasminoides var. Radicans Makino. |
