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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
田莹莹	TIAN Ying-ying	江西省中医药研究院中药研究所，江西南昌 330046	Department of Traditional Chinese Medicine, Jiangxi Provincial Institute of Traditional Chinese Medicine, Nanchang 330046, China
戴迪	DAI Di	江西省中医药研究院中药研究所，江西南昌 330046	Department of Traditional Chinese Medicine, Jiangxi Provincial Institute of Traditional Chinese Medicine, Nanchang 330046, China
张金莲	ZHANG Jin-lian	江西中医药大学，江西南昌 330004	Jiangxi University of Chinese Medicine, Nanchang 330004, China
陈超	CHEN Chao	江西省中医药研究院中药研究所，江西南昌 330046 国家中药材产业技术体系南昌综合试验站，江西南昌 330046 江西道地特色中药材标准化重点研究室，江西南昌 330046	Department of Traditional Chinese Medicine, Jiangxi Provincial Institute of Traditional Chinese Medicine, Nanchang 330046, China Nanchang Comprehensive Experimental Station of National Technology System of Chinese Medicinal Materials Industry, Nanchang 330046, China Jiangxi Key Laboratory of Standardization of Authentic Characteristic Chinese Medicinal Materials, Nanchang 330046, China
何小群	HE Xiao-qun	江西省中医药研究院中药研究所，江西南昌 330046 国家中药材产业技术体系南昌综合试验站，江西南昌 330046 江西道地特色中药材标准化重点研究室，江西南昌 330046	Department of Traditional Chinese Medicine, Jiangxi Provincial Institute of Traditional Chinese Medicine, Nanchang 330046, China Nanchang Comprehensive Experimental Station of National Technology System of Chinese Medicinal Materials Industry, Nanchang 330046, China Jiangxi Key Laboratory of Standardization of Authentic Characteristic Chinese Medicinal Materials, Nanchang 330046, China
谢茵	XIE Yin	江西省中医药研究院中药研究所，江西南昌 330046	Department of Traditional Chinese Medicine, Jiangxi Provincial Institute of Traditional Chinese Medicine, Nanchang 330046, China
黄兴明	HUANG Xing-ming	江西信前胡中药开发有限公司，江西上饶 334300	Jiangxi Xinqianhu Traditional Chinese Medicine Development Co., Ltd., Shangrao 334300, China
李晶*	LI Jing	江西省中医药研究院中药研究所，江西南昌 330046	Department of Traditional Chinese Medicine, Jiangxi Provincial Institute of Traditional Chinese Medicine, Nanchang 330046, China

基金项目:财政部和农业农村部：国家现代农业产业技术体系资助项目；江西省中医药标准化技术委员会2021年度第一批标准化项目（2021A06）；江西省药品监督管理局科研项目（2022JS35）；江西省中医药中青年骨干人才（第一批）培养计划（赣中医药科教字〔2020〕20号）

中文摘要:目的考察栽培方式、产地、加工方法及外观性状对前胡中香豆素类成分含量的影响。方法采用超高效液相色谱法，测定不同生长模式（大田栽培、山地栽培和山地野生）、产地、加工方法（“统个”、薄片、不同干燥温度）及外观性状（根部分叉或不分叉）前胡中白花前胡香豆精Ⅱ、白花前胡甲素、白花前胡乙素、白花前胡素E质量分数及4个香豆素质量分数的和（TC）。ACQUITY UPLC􀳏 BEH C₁₈色谱柱（100 mm×2.1 mm，1.7 μm）；流动相A为甲醇-四氢呋喃（60∶40）、流动相B为超纯水，梯度洗脱（0~25 min，40%~65%A）；检测波长为321 nm；柱温为30 ℃；流速为0.25 mL·min^–1；进样量为2 μL。结果 TC为山地野生>山地栽培>大田栽培；不同产地前胡的香豆素含量差异有统计学意义；不同干燥方法加工的前胡香豆素含量差异有统计学意义；根部分叉的前胡中白花前胡甲素含量显著低于不分叉的前胡。结论前胡生产和加工过程中，应选择山地栽培，尽可能减少根部分叉，加工应选择50 ℃以下“统个”干燥，该方法可以显著提高前胡中香豆素类成分的含量。

中文关键词:前胡生产加工香豆素影响因素

Influencing Factors of Coumarin Content in Peucedani Radix during Production and Processing

Abstract:Objective To investigate the effects of cultivation methods, producing areas, processing methods, and root bifurcation on the content of coumarins in Peucedani Radix.Methods The content of peucedanocoumarin Ⅱ (PⅡ), praeruptorin A (PA), praeruptorin B (PB), and praeruptorin E (PE) and the total content (TC) of the 4 coumarins in Peucedani Radix produced with different growth patterns (field planting, mountain planting, and mountain wild growth), different producing areas, different processing methods (intact roots, thin pieces, dried at different temperatures), and different root bifurcation traits (bifurcated or non-bifurcated roots) were determined by ultra-high performance liquid chromatography. The gradient elution was carried out in ACQUITY UPLC􀳏 BEH C₁₈ column (100 mm×2.1 mm, 1.7 μm) with mobile phase A of methanol-tetrahydrofuran (60:40) and mobile phase B of ultra-pure water in the procedure of 0-25 min, 40%-65%A. The detection wavelength, column temperature, flow rate, and injection volume were 321 nm, 30 ℃, 0.25 mL·min^–1, and 2 μL, respectively.Results TC followed the trend of mountain wild growth>mountain planting>field planting. The content of coumarins in Peucedani Radix had significant differences among different producing areas and different processing methods. The content of PA in bifurcated roots was significantly lower than that in non-bifurcated roots.Conclusion In the production and processing of Peucedani Radix, mountain planting, non-bifurcation of roots, and drying intact roots below 50 ℃could significantly increase the content of coumarins in Peucedani Radix.

keywords:Peucedani Radix production and processing coumarin influencing factor

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