| 滇鸡血藤红色素的提取工艺优化及其抑制COX-2活性研究 |
| 投稿时间：2023-12-18 点此下载全文
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| 引用本文：乔子璇,姜丹,任广喜,吴菲,李萌,华国栋,刘春生.滇鸡血藤红色素的提取工艺优化及其抑制COX-2活性研究[J].中国现代中药,2024,26(9):1570-1577 |
| DOI：10.13313/j.issn.1673-4890.20231218002 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 乔子璇 |
QIAO Zi-xuan |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 姜丹 |
JIANG Dan |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 任广喜 |
REN Guang-xi |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 吴菲 |
WU Fei |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 李萌 |
LI Meng |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 华国栋* |
HUA Guo-dong |
北京中医药大学 东直门医院，北京 100700 |
Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China |
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| 刘春生* |
LIU Chun-sheng |
北京中医药大学 中药学院，北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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| 基金项目:中央高校横向课题（2020110031001469） |
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| 中文摘要:目的 研究滇鸡血藤红色素的最佳提取条件，考察其基本性质与环氧化酶-2（COX-2）抑制活性，为滇鸡血藤的临床用药及相关制剂开发提供参考。方法 以吸光度为指标，采用单因素试验和正交试验对滇鸡血藤红色素提取工艺中的液料比、浸提时间、乙醇体积分数进行优化，确定滇鸡血藤红色素的最佳提取工艺，对提取得到的红色素光、热、pH稳定性进行考察，进一步采用COX-2抑制剂筛选试剂盒考察滇鸡血藤红色素对COX-2的抑制作用。结果 在50%乙醇、液料比为110、浸提12 h的条件下提取的滇鸡血藤红色素吸光度最大；其在室温避光、pH 1~9条件下有较好的稳定性；滇鸡血藤红色素对COX-2有较好的抑制作用，半数抑制浓度（IC50）为2.04 ng·mL–1（生药质量浓度）。结论 明确了滇鸡血藤红色素的最佳提取条件和稳定性条件，可为滇鸡血藤资源的综合开发利用与临床用药研究提供参考。 |
| 中文关键词:滇鸡血藤 红色素 环氧化酶-2 抑制率 |
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| Extraction of Red Pigment with Inhibitory Effect on COX-2 from Kadsurae Caulis |
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| Abstract:Objective To optimize the extraction conditions of red pigment from Kadsurae Caulis, examine the basic properties and the inhibitory effect of the red pigment on cyclooxygenase-2 (COX-2), and provide a reference for the clinical application of Kadsurae Caulis and the development of related preparations.Methods With absorbance as the indicator, single factor and orthogonal experiments were carried out to optimize the liquid-to-solid ratio, extraction time, and ethanol concentration for the extraction of red pigment from Kadsurae Caulis. Furthermore, the light, thermal, and pH stability of the red pigment was examined, and the COX-2 Inhibitor Screening Kit was used to determine the inhibitory effect of the red pigment on COX-2.Results The absorbance of the red pigment was the largest when the extraction was carried out with 50% ethanol and at the liquid-to-solid ratio of 110 for 12 h. The extracted pigment had good stability at room temperature and pH 1-9. Moreover, the extracted pigment inhibited the activity of COX-2, with the half maximal inhibitory concentration (IC50) of only 2.04 ng·mL–1 crude drug.Conclusion This study optimized the extraction conditions and examined the stability of red pigment from Kadsurae Caulis. The extracted red pigment demonstrated strong inhibitory effect on COX-2. The findings lay a foundation for the comprehensive utilization and development of the resources and the clinical application of Kadsurae Caulis. |
| keywords:Kadsurae Caulis red pigment COX-2 inhibition rate |
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