| 沙氏鹿茸草根腐病致病菌的生物学特性及室内药剂筛选 |
| 投稿时间：2024-09-18 修订日期：2024-10-22 点此下载全文
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| 中文摘要:为明确沙氏鹿茸草根腐病致病菌的生物学特性,筛选有效防治根腐病的化学杀菌剂和生物源杀菌剂,为防治沙氏鹿茸草根腐病害提供理论依据,本研究使用菌丝生长速率法研究了不同氮源、碳源、光照条件、培养基和pH对致病菌菌落生长的影响,并分别测定了10种化学杀菌剂和6种生物源杀菌剂对病原菌菌株的抑制效果。研究发现,鹿茸草根腐病致病菌生长的最适温度为30℃,最适pH为7.0,最适氮源为硝酸钠,最适碳源α-乳糖,最适光照条件为连续光照,最适培养基为Czapek’s和PDA。药剂筛选的结果表明,甲霜恶霉灵和乙蒜素对病原菌抑制效果较好,EC50分别为0.0540和19.081 mg.L-1。 |
| 中文关键词:沙氏鹿茸草 根腐病 共享镰孢菌 药剂筛选 乙蒜素 |
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| Biological Characterization and Determination of Fungicide Virulence of the Causal Agent of Root Rot of Monochasma savatieri |
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| Abstract:In order to clarify the biological properties of Fusarium commune, the causal agent of root rot of Monochasma savatieri, and to screen chemical fungicides and biogenic fungicides for effective control of root rot, and to provide theoretical basis for the prevention and control of root rot of Shah"s deer antler grass, in this study, the effects of different nitrogen and carbon sources, light conditions, culture medium and pH on the growth of the causal fungus were investigated by using the method of mycelial growth rate, and the inhibitory effects of 10 chemical fungicides and 6 biogenic fungicides on the pathogenic fungi were determined respectively. It was shown that the optimum temperature for the growth of root rot-causing fungi of Monochasma savatieri was 30°C, the optimum pH was 7.0, the optimum nitrogen source was sodium nitrate, the optimum carbon source was α-lactose, the optimum light condition was continuous light, and the optimum media were Czapek"s and PDA. The results of the pharmaceutical screening showed that the chemical fungicides and biogenic fungicides with better inhibitory effects on the pathogens were Difenocarb and Ethylicin, respectively, with EC50 of 0.0540 and 19.081 mg.L-1, and the inhibitory effect of Ethylicin was slightly worse than that of difenocarb. |
| keywords:Monochasma savatieri root rot Fusarium commune fungicide Screening Ethylicin |
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