| Abstract:Objective: The content of chemical components, antioxidant activity in vitro and antibacterial activity of volatile oil in different parts of Euodiae fructus were studied, so as to provide a theoretical basis for making full use of Evodia resources. Methods: Ultra-performance liquid chromatography (UPLC) was used to determine the contents of 10 chemical components in different parts (fruits, branches and leaves) of Evodia officinalis, and the volatile oil of Evodia fruit and leaves was extracted by steam distillation. The antioxidant activity of different parts (fruits, branches and leaves) of Evodia officinalis and the antibacterial activity of volatile oil in different parts (fruits and leaves) of Evodia officinalis were evaluated by DPPH removal method and carrier immersion quantitative antibacterial test. Results: Evodia leaves contained more phenolic acids, flavonoids and alkaloids, among which the chlorogenic acid content was as high as 4.1 mg·g-1, which was comparable to that of Euodiae fructus, and the hypericin content was as high as 8.8 mg·g-1, which was much higher than that of Euodiae fructus. The scavenging ability of Evodia leaves on DPPH free radicals was also comparable to that of fruits, and the volatile oil had strong inhibitory effects on Staphylococcus aureus, Escherichia coli and Candida albicans. The types and contents of chemical components contained in Evodia officinalis branches were relatively small, and the scavenging ability of DPPH free radicals was much lower than that of Evodia officinalis fruits and leaves.. Conclusion: In this paper, the chemical composition and in vitro pharmacological activity of the non-medicinal parts of Evodia officinalis were preliminarily explored, and it was found that the leaves of Evodia officinalis were rich in active ingredients, and had strong antioxidant capacity and antibacterial effect in vitro, which could provide a reference for the comprehensive utilization of Evodia resources. |
