| 温阳通络方基于miR-26b-3p调控类风湿关节炎滑膜成纤维细胞的机制研究 |
| 投稿时间：2024-02-29 点此下载全文
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| 引用本文：李冬艳,田亚丹,宋佳欣,罗定霞,吴晶金.温阳通络方基于miR-26b-3p调控类风湿关节炎滑膜成纤维细胞的机制研究[J].中国现代中药,2024,26(10):1719-1726 |
| DOI：10.13313/j.issn.1673-4890.20240229001 |
| 摘要点击次数: 44 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 李冬艳 |
LI Dong-yan |
云南中医药大学 第一临床医学院，云南 昆明 650011 |
The First Clinical Medical College of Yunnan University of Traditional Chinese Medicine, Kunming 650011, China |
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| 田亚丹 |
TIAN Ya-dan |
云南中医药大学 第一临床医学院，云南 昆明 650011 |
The First Clinical Medical College of Yunnan University of Traditional Chinese Medicine, Kunming 650011, China |
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| 宋佳欣 |
SONG Jia-xin |
云南中医药大学 第一临床医学院，云南 昆明 650011 |
The First Clinical Medical College of Yunnan University of Traditional Chinese Medicine, Kunming 650011, China |
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| 罗定霞 |
LUO Ding-xia |
云南中医药大学 第一临床医学院，云南 昆明 650011 |
The First Clinical Medical College of Yunnan University of Traditional Chinese Medicine, Kunming 650011, China |
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| 吴晶金* |
WU Jing-jin |
云南省中医医院 风湿科，云南 昆明 650021 |
Department of Rheumatology, Yunnan Provincial Hospital of Traditional Chinese Medicine, Kunming 650021, China |
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| 基金项目:国家自然科学基金项目（82360924）；云南省中医联合专项重点项目（202101AZ070001-161）；兴滇英才“青年人才”项目；云南省高层次中医痹病学后备人才项目 |
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| 中文摘要:目的 探讨温阳通络方基于微小RNA（miR）-26b-3p调控类风湿关节炎滑膜成纤维细胞增殖、迁移、侵袭的作用。方法 SD大鼠随机分为温阳通络方组和空白血清组。温阳通络方组大鼠给药剂量为4.05 g·kg–1·d–1，2次/d，间隔12 h，连续给药3 d，末次给药后1 h腹主动脉取血分离血清；细胞计数试剂盒-8（CCK-8）实验筛选温阳通络方含药血清最佳浓度和干预时间；在人类风湿关节炎滑膜成纤维细胞MH7A中分别转染inhibitor NC、miR-26b-3p inhibitor、mimic NC、miR-26b-3p mimic片段，并用温阳通络方含药血清干预，设置正常关节成纤维样滑膜细胞为对照组，实时定量聚合酶链式反应（qRT-PCR）检测MH7A细胞中miR-26b-3p的表达；CCK-8实验检测各组细胞的增殖情况；Transwell实验检测各组细胞的迁移、侵袭情况。结果 不同浓度温阳通络方含药血清均能上调miR-26b-3p的表达（P<0.05），miR-26b-3p的表达呈浓度依赖性升高。温阳通络方含药血清干预MH7A细胞后，细胞的增殖、迁移、侵袭能力减弱（P<0.05）。miR-26b-3p过表达后，MH7A细胞的增殖、迁移、侵袭能力减弱（P<0.05）；干扰miR-26b-3p的表达后，MH7A细胞的增殖、迁移、侵袭能力增强（P<0.05）。在干扰miR-26b-3p表达的基础上，温阳通络方含药血清干预MH7A细胞后，细胞的增殖、迁移、侵袭能力减弱（P<0.05）。结论 温阳通络方通过上调miR-26b-3p的表达抑制类风湿关节炎滑膜成纤维细胞的增殖、迁移、侵袭。 |
| 中文关键词:类风湿关节炎 微小RNA-26b-3p 温阳通络方 滑膜成纤维细胞 |
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| Mechanism of Wenyang Tongluo Prescription in Regulating Synovial Fibroblasts in Rheumatoid Arthritis Based on MiR-26b-3p |
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| Abstract:Objective To investigate the regulatory effect of Wenyang Tongluo Prescription on the proliferation, migration, and invasion of synovial fibroblasts in rheumatoid arthritis based on miR-26b-3p.Methods SD rats were randomly divided into a Wenyang Tongluo Prescription group and a blank serum group. The Wenyang Tongluo Prescription group was given Wenyang Tongluo Prescription at 4.05 g·kg–1·d–1, twice daily, with a 12-hour interval, for 3 consecutive days. Blood was collected from the abdominal aorta 1 hour after the last administration, followed by serum isolation. The Cell Counting Kit-8 (CCK-8) assay was used to determine the optimal concentration and intervention time of Wenyang Tongluo Prescription-containing serum. Human rheumatoid arthritis synovial fibroblasts (MH7A) were transfected with inhibitor NC, miR-26b-3p inhibitor, mimic NC, and miR-26b-3p mimic, respectively, and treated with Wenyang Tongluo Prescription-containing serum. Human fibroblast-like synovial cells (HFLS) were set as the blank control group. Real-time quantitative PCR (qRT-PCR) was used to detect the expression of miR-26b-3p in MH7A cells. The proliferation of cells in each group was detected by the CCK-8 assay. The migration and invasion of cells in each group were detected by the Transwell assay.Results The expression of miR-26b-3p was upregulated by serum containing Wenyang Tongluo Prescription at different concentrations (P<0.05), and the expression of miR-26b-3p increased in a concentration-dependent manner. After the intervention with the drug-containing serum of Wenyang Tongluo Prescription, the proliferation, migration, and invasion abilities of MH7A cells were weakened (P<0.05). After miR-26b-3p overexpression, the proliferation, migration, and invasion of MH7A cells were weakened (P<0.05). After interfering with the expression of miR-26b-3p, the proliferation, migration, and invasion of MH7A cells were enhanced (P<0.05). On the basis of interfering with the expression of miR-26b-3p, the proliferation, migration, and invasion of MH7A cells were weakened after intervention with the drug-containing serum of Wenyang Tongluo Prescription (P<0.05).Conclusion Wenyang Tongluo Prescription can inhibit the proliferation, migration, and invasion of synovial fibroblasts in rheumatoid arthritis by upregulating the expression of miR-26b-3p. |
| keywords:rheumatoid arthritis miR-26b-3p Wenyang Tongluo Prescription synovial fibroblasts |
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