| 川芎SSR标记开发及遗传多样性分析 |
| 投稿时间：2024-03-01 点此下载全文
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| 引用本文：毛常清,沙秀芬,陶珊,吴宇,彭芳,廖海浪,袁灿,张超.川芎SSR标记开发及遗传多样性分析[J].中国现代中药,2024,26(11):1854-1866 |
| DOI：10.13313/j.issn.1673-4890.20240301003 |
| 摘要点击次数: 295 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 毛常清 |
MAO Chang-qing |
四川省农业科学院 经济作物研究所，四川 成都 610300 |
Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu610300, China |
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| 沙秀芬 |
SHA Xiu-fen |
四川省农业科学院 经济作物研究所，四川 成都 610300 |
Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu610300, China |
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| 陶珊 |
TAO Shan |
四川省农业科学院 经济作物研究所，四川 成都 610300 |
Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu610300, China |
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| 吴宇 |
WU Yu |
四川省农业科学院 经济作物研究所，四川 成都 610300 |
Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu610300, China |
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| 彭芳 |
PENG Fang |
四川省农业科学院 经济作物研究所，四川 成都 610300 |
Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu610300, China |
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| 廖海浪 |
LIAO Hai-lang |
四川省农业科学院 经济作物研究所，四川 成都 610300 |
Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu610300, China |
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| 袁灿* |
YUAN Can |
四川省农业科学院 经济作物研究所，四川 成都 610300 |
Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu610300, China |
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| 张超* |
ZHANG Chao |
四川省农业科学院 经济作物研究所，四川 成都 610300 |
Industrial Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu610300, China |
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| 基金项目:财政部和农业农村部：国家现代农业产业技术体系资助项目；四川省科技计划项目（2021YFYZ0012）；四川省财政自主创新专项（2022ZZCX076） |
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| 中文摘要:目的 基于川芎基因组调研结果开发简单重复序列（SSR）标记，对109份川芎种质资源进行遗传多样性分析，为川芎种质资源评价、保存和新品种选育等提供理论依据。方法 基于Survey测序已组装序列挖掘川芎基因组中的SSR位点并设计引物，筛选多态性高的引物，对109份川芎种质资源进行遗传多样性和遗传分化分析，并在川芎及其近缘物种中进行通用性验证。结果 36对引物在109份川芎种质资源中共检测到102个等位基因位点，观测等位基因数为2.833 3，期望杂合度为0.488 5，Shannon's指数为0.807 1，Nei基因多样性为0.486 0，多态信息含量为0.419 4，采集的109份川芎种质资源具有丰富的遗传多样性。基于遗传距离的聚类分析显示，在遗传相似系数为0.76处，109份川芎聚为两大类，但其与地理分布关系不大。通用性验证显示，36对SSR引物在川芎及其近缘物种中通用性较好。结论 开发的36对SSR标记能很好地揭示川芎的遗传多样性，供试109份川芎种质遗传分化小、基因流较大。 |
| 中文关键词:川芎 简单重复序列标记 遗传多样性 遗传分化 |
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| Development of SSR Markers for Ligusticum chuanxiong and Its Genetic Diversity Analysis |
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| Abstract:Objective Simple sequence repeat (SSR) markers of Ligusticum chuanxiong were developed with its genome survey results to analyze the genetic diversity of 109 L. chuanxiong germplasm resources. This study can provide theoretical basis for the evaluation and preservation of L. chuanxiong germplasm resources and new variety breeding.Methods SSR loci in the genome of L. chuanxiong were identified based on sequences assembled in Survey sequencing, and primers were designed. The primers with high polymorphisms were screened to analyze the genetic diversity and genetic differentiation of 109 L. chuanxiong germplasm resources, and universality was verified among L. chuanxiong and its closely related species.Results A total of 102 allelic loci were detected by 36 pairs of primers in 109 L. chuanxiong germplasm resources with the observed allele number of 2.833 3, the expected heterozygosity of 0.488 5, the Shannon's index of 0.807 1, the Nei's gene diversity of 0.486 0, and the polymorphic information content of 0.419 4. The 109 L. chuanxiong samples collected exhibited abundant genetic diversity. A cluster analysis based on genetic distance showed that the 109 L. chuanxiong samples were clustered into two major groups at a genetic similarity coefficient of 0.76, but it was not associated with geographical distribution. The universality validation showed that the 36 pairs of SSR primers had good universality among L. chuanxiong and its closely related species.Conclusion The 36 pairs of SSR markers developed were able to reveal the genetic diversity of L. chuanxiong well, and the 109 L. chuanxiong germplasm resources have small genetic differentiation and large gene flow. |
| keywords:Ligusticum chuanxiong Hort. SSR markers genetic diversity genetic differentiation |
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