| 人肠道菌群对党参水提物的体外转化作用 |
| 投稿时间：2024-04-19 点此下载全文
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| 引用本文：吴孟娇,李涵艺,聂宇迪,杨澜,王如峰.人肠道菌群对党参水提物的体外转化作用[J].中国现代中药,2024,26(11):1919-1929 |
| DOI：10.13313/j.issn.1673-4890.20240419005 |
| 摘要点击次数: 276 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 吴孟娇 |
WU Meng-jiao |
北京中医药大学 生命科学学院，北京 102488 |
School of Life Sciences, Beijing University of Chinese Medicine, Beijing102488, China |
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| 李涵艺 |
LI Han-yi |
北京中医药大学 生命科学学院，北京 102488 |
School of Life Sciences, Beijing University of Chinese Medicine, Beijing102488, China |
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| 聂宇迪 |
NIE Yu-di |
北京中医药大学 生命科学学院，北京 102488 |
School of Life Sciences, Beijing University of Chinese Medicine, Beijing102488, China |
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| 杨澜 |
YANG Lan |
北京中医药大学 生命科学学院，北京 102488 |
School of Life Sciences, Beijing University of Chinese Medicine, Beijing102488, China |
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| 王如峰* |
WANG Ru-feng |
北京中医药大学 生命科学学院，北京 102488 |
School of Life Sciences, Beijing University of Chinese Medicine, Beijing102488, China |
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| 基金项目:国家重点基础研究发展计划项目（2022YFC3501704） |
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| 中文摘要:目的 观察人肠道菌群对党参水提物的体外转化作用。方法 将党参水提液与人肠道菌群在体外实验条件下共孵育，采用高效液相色谱法对不同时间点样品中的紫丁香苷、党参苷Ⅰ、党参炔苷宁、党参炔苷进行含量测定；采用超高效液相色谱-四极杆-静电轨道场离子阱质谱法（UHPLC-Q-Exactive Orbitrap MS）分别对孵育0、24 h的样品进行化学成分定性分析。结果 定量分析结果表明，党参苷Ⅰ的含量在孵育24 h内没有明显变化；孵育2 h，紫丁香苷、党参炔苷宁、党参炔苷开始被转化；孵育24 h样品中，被转化的3个化合物的平均含量变化率在65%以上。定性分析结果表明，共鉴定出163个化合物，其中差异代谢物有53个。结论 体外条件下，人肠道菌群对紫丁香苷、党参炔苷宁、党参炔苷有明显的转化作用，对党参苷Ⅰ没有转化作用。转化前后，党参水提物的组成存在差异。 |
| 中文关键词:党参水提物 肠道菌群 生物转化 高效液相色谱法 |
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| In Vitro Transformation of Codonopsis pilosula Aqueous Extract by Human Intestinal Flora |
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| Abstract:Objective To observe the in vitro transformation of Codonopsis pilosula aqueous extract by human intestinal flora.Methods C. pilosula aqueous extract was incubated with human intestinal flora under in vitro experimental conditions. High-performance liquid chromatography (HPLC) was used to measure the content of syringin, tangshenoside Ⅰ, lobetyolinin, and lobetyolin at different time points. Ultra-high-performance liquid chromatography-Q-Exactive Orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap MS) was employed for qualitative analysis of the chemical components in the samples after 0 and 24 hours of incubation.Results Quantitative analysis showed that the content of tangshenoside Ⅰ did not significantly change within 24 h of incubation. Syringin, lobetyolinin, and lobetyolin began to be transformed after 2 h of incubation, and more than 65% of these three compounds were transformed after 24 h. Qualitative analysis identified 163 compounds in total, including 53 differential metabolites.Conclusion Under in vitro conditions, human intestinal flora significantly transforms syringin, lobetyolinin, and lobetyolin, but not tangshenoside Ⅰ. The composition of C. pilosula aqueous extract differs before and after transformation. |
| keywords:Codonopsis pilosula aqueous extract intestinal flora biotransformation HPLC |
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