| 基于肠道菌群-短链脂肪酸途径的人参改善运动性疲劳作用机制探索 |
| 投稿时间：2024-03-27 点此下载全文
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| 引用本文：刘晶晶,金红宇,胡晓茹,刘静,汪祺,徐蓓蕾,左甜甜,魏锋.基于肠道菌群-短链脂肪酸途径的人参改善运动性疲劳作用机制探索[J].中国现代中药,2024,26(11):1930-1937 |
| DOI：10.13313/j.issn.1673-4890.20240327004 |
| 摘要点击次数: 293 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 刘晶晶 |
LIU Jing-jing |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing100050, China |
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| 金红宇 |
JIN Hong-yu |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing100050, China |
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| 胡晓茹 |
HU Xiao-ru |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing100050, China |
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| 刘静 |
LIU Jing |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing100050, China |
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| 汪祺 |
WANG Qi |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing100050, China |
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| 徐蓓蕾 |
XU Bei-lei |
哈尔滨商业大学，黑龙江 哈尔滨 150000 |
Harbin University of Commerce, Harbin150000, China |
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| 左甜甜* |
ZUO Tian-tian |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing100050, China |
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| 魏锋* |
WEI Feng |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing100050, China |
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| 中文摘要:目的 探索人参提取物通过调节肠道菌群发挥抗疲劳作用的机制。方法 制备大鼠负重游泳运动性疲劳模型，连续灌胃给予人参提取物21 d。采用负重游泳实验评价人参提取物抗疲劳作用；收集大鼠血清、肠道内容物及各组织，采用紫外-可见分光光度法测定人参提取物中总皂苷和总多糖含量；采用比色法检测尿素氮、乳酸和肝/肌糖原水平；采用酶联免疫吸附法检测白细胞介素-6（IL-6）、IL-1β和C反应蛋白（CRP）水平；采用16S rRNA结合多元统计分析探索肠道菌群变化；采用液相色谱-质谱法（LC-MS/MS）测定血清短链脂肪酸变化。结果 人参提取物中总皂苷和总多糖质量分数分别为205.6、321.8 mg·g–1。与模型组比较，人参提取物给药后能够显著延长大鼠负重游泳时间，同时上调肝脏和骨骼肌组织中糖原水平，下调血清中尿素氮和乳酸水平。与模型组比较，人参提取物可促进生成短链脂肪酸的肠道菌群表达，并提高血清中短链脂肪酸水平，同时下调炎症因子水平。结论 人参提取物具有明显的抗疲劳作用，其机制可能与调控肠道菌群促进短链脂肪酸生成，进而促进糖原生成，同时抑制炎症因子表达有关。 |
| 中文关键词:人参 抗疲劳 肠道菌群 短链脂肪酸 炎症因子 |
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| Mechanism of Panax ginseng in Improving Exercise Fatigue Based on Gut Microbiota-Short-chain Fatty Acid Pathway |
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| Abstract:Objective To explore the mechanism of Panax ginseng extract in anti-fatigue through the regulation of the gut microbiota.Methods A rat model of exercise-induced fatigue was established by forced load swimming, and P. ginseng extract was administered by gavage for 21 days. The anti-fatigue effect was evaluated through the forced load swimming test, and rat serum, intestinal contents, and various tissues were collected. The total ginsenosides and total polysaccharide content in ginseng extract were determined using UV-spectrophotometry. Colorimetric methods were used to detect urea nitrogen, lactate, and hepatic/muscle glycogen levels. Enzyme-linked immunosorbent assay was used to measure interleukin-6 (IL-6), interleukin-1β (IL-1β), and C-reactive protein (CRP) levels, and 16S rRNA combined with multivariate statistical analysis was employed to explore changes in the gut microbiota. Furthermore, LC-MS/MS was used to measure changes in serum short-chain fatty acids.Results The total ginsenosides and total polysaccharide content in ginseng extract were found to be 205.6 and 321.8 mg·g–1 of extract, respectively. Compared with the model group, administration of P. ginseng extract significantly prolonged the forced load swimming time of rats, upregulated glycogen levels in the liver and skeletal muscle tissues, and downregulated urea nitrogen and lactate levels in the serum. P. ginseng extract promoted the expression of gut microbiota involved in the production of short-chain fatty acids, increased serum short-chain fatty acid levels, and reduced levels of inflammatory factors compared with the model group.Conclusion P. ginseng extract exhibits a significant anti-fatigue effect, which may be achieved by regulating the gut microbiota to promote the generation of short-chain fatty acids, thereby promoting glycogen synthesis while inhibiting inflammatory factors. |
| keywords:Panax ginseng C.A.Mey. anti-fatigue gut microbiota short-chain fatty acids inflammatory factor |
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