| 藏药十味乳香散HPLC特征图谱及5个成分的含量测定 |
| 投稿时间：2024-04-19 点此下载全文
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| 引用本文：吴明慧,江德恩,杨俊峰,王佩,袁志兵,谢和兵.藏药十味乳香散HPLC特征图谱及5个成分的含量测定[J].中国现代中药,2024,26(11):1980-1988 |
| DOI：10.13313/j.issn.1673-4890.20240419003 |
| 摘要点击次数: 289 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 吴明慧 |
WU Ming-hui |
安徽中医药大学 药学院，安徽 合肥 230012
南通市海门长三角药物高等研究院，江苏 南通 226133
江苏神猴医药研究有限公司，江苏 南通 226133 |
School of Pharmacy, Anhui University of Chinese Medicine, Hefei230012, China
Nantong Haimen Yangtze Delta Drug Advanced Research Institute, Nantong226133, China
Jiangsu Shenhou Pharmaceutical Research Co., Ltd., Nantong226133, China |
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| 江德恩 |
JIANG De-en |
安徽中医药大学 药学院，安徽 合肥 230012
南通市海门长三角药物高等研究院，江苏 南通 226133
江苏神猴医药研究有限公司，江苏 南通 226133 |
School of Pharmacy, Anhui University of Chinese Medicine, Hefei230012, China
Nantong Haimen Yangtze Delta Drug Advanced Research Institute, Nantong226133, China
Jiangsu Shenhou Pharmaceutical Research Co., Ltd., Nantong226133, China |
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| 杨俊峰 |
YANG Jun-feng |
安徽中医药大学 药学院，安徽 合肥 230012
南通市海门长三角药物高等研究院，江苏 南通 226133
江苏神猴医药研究有限公司，江苏 南通 226133 |
School of Pharmacy, Anhui University of Chinese Medicine, Hefei230012, China
Nantong Haimen Yangtze Delta Drug Advanced Research Institute, Nantong226133, China
Jiangsu Shenhou Pharmaceutical Research Co., Ltd., Nantong226133, China |
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| 王佩 |
WANG Pei |
安徽中医药大学 药学院，安徽 合肥 230012
南通市海门长三角药物高等研究院，江苏 南通 226133
江苏神猴医药研究有限公司，江苏 南通 226133 |
School of Pharmacy, Anhui University of Chinese Medicine, Hefei230012, China
Nantong Haimen Yangtze Delta Drug Advanced Research Institute, Nantong226133, China
Jiangsu Shenhou Pharmaceutical Research Co., Ltd., Nantong226133, China |
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| 袁志兵* |
YUAN Zhi-bing |
安徽中医药大学 药学院，安徽 合肥 230012
南通市海门长三角药物高等研究院，江苏 南通 226133
江苏神猴医药研究有限公司，江苏 南通 226133 |
School of Pharmacy, Anhui University of Chinese Medicine, Hefei230012, China
Nantong Haimen Yangtze Delta Drug Advanced Research Institute, Nantong226133, China
Jiangsu Shenhou Pharmaceutical Research Co., Ltd., Nantong226133, China |
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| 谢和兵* |
XIE He-bing |
安徽中医药大学 药学院，安徽 合肥 230012
南通市海门长三角药物高等研究院，江苏 南通 226133
江苏神猴医药研究有限公司，江苏 南通 226133
西藏神猴药业有限责任公司，西藏 日喀则 857000 |
School of Pharmacy, Anhui University of Chinese Medicine, Hefei230012, China
Nantong Haimen Yangtze Delta Drug Advanced Research Institute, Nantong226133, China
Jiangsu Shenhou Pharmaceutical Research Co., Ltd., Nantong226133, China
Xizang Shenhou Pharmaceutical Co., Ltd., Rikaze857000, China |
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| 基金项目:国家自然科学基金项目（81470247）；西藏自治区科技厅区域科技协同创新专项（QYXTZX-RKZ2022-07） |
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| 中文摘要:目的 建立藏族药十味乳香散高效液相色谱法（HPLC）特征图谱，以及指标成分没食子酸、诃子联苯酸、鞣花酸、诃子酸、11-羰基-β-乙酰乳香酸含量测定方法。方法 采用Ultimate XB-C18色谱柱，以0.1%磷酸水溶液（A）-乙腈（B）为流动相，梯度洗脱，流速为1.0 mL·min–1，检测波长为250 nm，柱温为30 ℃，进样量为10 μL。结果 建立的15批十味乳香散特征图谱中标识出25个共有峰，相似度均大于0.992；指标成分没食子酸、诃子联苯酸、鞣花酸、诃子酸和11-羰基-β-乙酰乳香酸的线性范围分别为2.86~57.20（r=1.000 0）、8.18~163.54（r=0.999 9）、3.61~72.21（r=0.999 7）、9.96~199.23（r=0.999 7）、0.53~10.69 μg·mL–1（r=0.999 9）。稳定性、精密度、重复性均较好，平均加样回收率分别为100.6%（RSD=0.90%）、100.5%（RSD=2.14%）、103.6%（RSD=1.19%）、101.0%（RSD=1.22%）、102.2%（RSD=2.54%）。结论 建立的特征图谱和指标成分含量测定方法准确、简便、灵敏度高，可为提升十味乳香散的质量标准提供参考。 |
| 中文关键词:十味乳香散 高效液相色谱法 含量测定 特征图谱 |
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| HPLC Specific Chromatogram of Tibetan Medicine Shiwei Ruxiang Powder and Content Determination of Five Components |
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| Abstract:Objective To establish high performance liquid chromatography (HPLC) specific chromatogram for the Tibetan medicine Shiwei Ruxiang Powder and a method for determining the content of index components: gallic acid, chebulagic acid, ellagic acid, chebulinic acid, and acetyl-11-keto-β-boswellic acid.Methods Gradient elution was carried out in the Ultimate XB-C18 column with 0.1% phosphoric acid aqueous solution (A)-acetonitrile (B) as the mobile phase at a flow rate of 1.0 mL·min–1. The detection wavelength was 250 nm, and the column temperature was 30 ℃. The sample injection volume was 10 μL.Results Twenty-five common peaks were identified in the specific chromatograms of 15 batches of Shiwei Ruxiang Powder, with the similarities more than 0.992. The linear ranges of gallic acid, chebulagic acid, ellagic acid, chebulinic acid, and acetyl-11-keto-β-boswellic acid were 2.86-57.20 (r=1.000 0), 8.18-163.54 (r=0.999 9), 3.61-72.21 (r=0.999 7), 9.96-199.23 (r=0.999 7), and 0.53-10.69 μg·mL–1 (r=0.999 9), respectively. The relative standard deviations (RSD) of stability, precision. The average recovery of the index components was 100.6% (RSD=0.90%), 100.5% (RSD=2.14%), 103.6% (RSD=1.19%), 101.0% (RSD=1.22%), and 102.2% (RSD=2.54%), respectively.Conclusion The established specific chromatogram and index component content determination method are precise, simple, and sensitive, which can provide a reference for improving the quality standard of Shiwei Ruxiang Powder. |
| keywords:Shiwei Ruxiang Powder HPLC content determination characteristic chromatogram |
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