| Abstract:Objective To evaluate the therapeutic effect of Shenling Baizhu Powder on diabetes obesity by literature meta-analysis, and to analyze the mechanism of Shenling Baizhu Powder on diabetes obesity by combining network pharmacology and animal experiment methods. Methods Relevant domestic and foreign literatures were included. BMI, HbA1c, 2h postprandial blood glucose (2hPG), fasting blood glucose (FBG), Home-IR, waist hip ratio were used as outcome indicators to evaluate the therapeutic effect of Shenling Baizhu Powder on diabetes obesity; Download the genes related to diabetes obesity from GEO chip, download the action targets of Shenling Baizhu Powder in TCMSP, TCMID and BATMAN-TCM, analyze the protein interaction between the intersection targets of the two, and then screen out the core target proteins according to topological analysis; The model of diabetes obese mice was established. After grouping, the mice were treated with Shenling Baizhu Powder by gavage. Two weeks later, the body weight, blood sugar, Lee's index of each group of mice were measured. The volume changes of adipocytes in each group of mice were analyzed with the Hematoxylin eosin method, and the concentrations of cholesterol, triglyceride and core target protein in mouse serum were measured. A total of 9 articles were included, with complete data and no publication bias found. The evaluation was low risk. After taking Shen Ling Bai Zhu San, the patient's BMI, HbA1c, 2-hour PGFBG, Homea IR, and waist to hip ratio were significantly improved; There are 337 genes related to obesity in diabetes, 2067 action targets and 59 intersection targets of Shenling Baizhu Powder, 7 signaling pathways related to obesity in diabetes, including Biosynthesis of unsaturated fat acids, Fat acid metabolism, and the core targets are APO-E, IGF-1, PAI-1; The results of animal experiments showed that after oral administration of Shenling Baizhu San, the blood glucose levels in mice were not significantly changed, while body weight and Lee's index showed a significant downward trend. Adipose cells were significantly reduced, while cholesterol and triglyceride content, APO-E, IGF-1, and PAI-1 protein concentrations showed a downward trend. Conclusion According to the results of meta-analysis and animal experiments, Shenling Baizhu Powder has obvious therapeutic effect on diabetes and obesity, and its mechanism may be related to influencing the synthesis of unsaturated fatty acids and fatty acids, reducing the transformation of sugar and fat, correcting the imbalance of energy metabolism, and up regulating autophagy to promote the decomposition of adipose tissue. |
