| 3,6?-二芥子酰基蔗糖神经保护作用研究 |
| 投稿时间：2024-02-20 修订日期：2024-04-18 点此下载全文
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| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要:目的：研究中药远志中寡糖酯类成分3,6?-二芥子酰基蔗糖（DISS）对神经的保护作用。方法：将不同浓度的DISS与APP-SH-SY5Y细胞共同孵育24 h，MTT法检测各组细胞的存活率，免疫荧光法检测细胞内活性氧（ROS）水平，生化法检测超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性，ELISA法检测细胞内和培养液中β-淀粉样蛋白（Aβ）含量，蛋白质免疫印迹法（Western blotting）法检测细胞内淀粉样前体蛋白（APP）、微管相关蛋白轻链3-Ⅰ（LC3-Ⅰ）和LC3-Ⅱ的表达。结果：DISS可以提高APP-SH-SY5Y细胞的存活率（P<0.05），减少细胞内ROS的生成（P<0.01），提高SOD和GSH-Px活力（P<0.05），降低细胞内和培养液中的Aβ含量（P<0.01，P<0.05），明显增加细胞内LC3Ⅱ蛋白表达水平，增加LC3Ⅱ/LC3Ⅰ水平（P<0.05），对APP表达无明显影响。结论：DISS可能通过降低细胞内氧化应激，减少细胞Aβ表达及调节自噬发挥神经保护作用。 |
| 中文关键词:3,6’-二芥子酰基蔗糖 APP-SH-SY5Y细胞 阿尔茨海默病 β淀粉样蛋白 氧化应激 自噬 |
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| Studies on Neuroprotective Effect of 3,6'-Disinapoyl sucrose |
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| Abstract:Objective: To study the neuroprotective effects of 3,6'-disinapoyl sucrose (DISS), an oligosaccharide ester component of the traditional Chinese medicine Polygala tenuifolia Willd. Methods: Different concentrations of DISS were co-incubated with APP-SH-SY5Y cells for 24 h. The survival rate was detected by MTT., The intracellular reactive oxygen species (ROS) levels were detected by immunofluorescence. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px)were detected by biochemical methods. The intracellular and culture medium contents of β-amyloid protein (Aβ) level were detected by ELISA. The expression of APP, microtubule-associated protein lightchain 3-Ⅰ(LC3-Ⅰ) and microtubule-associated protein lightchain 3-Ⅱ(LC3-Ⅱ) were detected by western blotting. Results: DISS could significantly increase the viability of APP-SH-SY5Y cells (P<0.05), decrease the production of intracellular ROS (P < 0.01), and increase the activities of SOD and GSH-px in APP-SH-SY5Y cells (P < 0.05). Meanwhile, DISS also significantly decreased the Aβ content in APP-SH-SY5Y cells (P < 0.01) and cell culture medium (P < 0.05) , increased the expression of intracellular LC3Ⅱ protein and promoted the ratio of LC3Ⅱ/LC3Ⅰ(P < 0.05). However, DISS had no significant effect on APP expression. Conclusion: DISS may exert neuroprotective effects by reducing intracellular oxidative stress, decreasing cellular Aβ expression and regulating autophagy. |
| keywords:3,6'-Disinapoyl sucrose APP-SH-SY5Y cells Alzheimer's disease β-Amyloid protein oxidative stress autophagy |
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