RP-HPLC同时测定野菊花中绿原酸、木犀草苷和蒙花苷的含量 |
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引用本文:郭美兰,敬应春,蔡国琴.RP-HPLC同时测定野菊花中绿原酸、木犀草苷和蒙花苷的含量[J].中国现代中药,2012,14(4):10-13 |
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中文摘要:建立同时测定野菊花中绿原酸、木犀草苷和蒙花苷3个主要活性成分含量的方法。方法:采用RP-HPLC法,选用Kromasil-C18(250 mm×4.6 mm,5 μm,)色谱柱;以乙腈-0.05%磷酸溶液为流动相(梯度洗脱);流速为1.0 mL·min-1;检测波长为334 nm;柱温为30℃。结果:绿原酸、木犀苷和蒙花苷分别在40.44~647.04 ng(r=0.999 8)、19.50~311.92 ng(r=0.999 7)和253.62~405 7.82 ng(r=0.999 9)范围内线性关系良好;平均回收率分别为100.96%、99.99%和98.94%,RSD均<2.0%。22批野菊花药材中绿原酸含量为0.108%~0.545%,木犀苷含量为0.029%~0.393%,蒙花苷含量为0.028%~3.518%。结论:本方法简便准确、稳定可靠,可以用于野菊花药材的含量测定。 |
中文关键词:野菊花 RP-HPLC法 绿原酸 木犀草苷 蒙花苷 |
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RP-HPLC Simultaneous Determination of Chlorogenic Acid,luteolin-7-O-β-D-glucoside and Linarin in Chrysanthemi Indici Flos |
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Abstract:To establish a method for the determination of three active ingredients,chlorogenic acid,luteolin-7-O-β-D-glucoside,and Linarin in Flos Chrysanthemi Indici. Methods: A Kromasil -C18(250 mm×4.6 mm, 5 μm)column was used with the mobile phase (gradient elution) being acetonitrile-0.05% phosphate acid , flow rate being 1.0 mLomin-1,detecting wavelength being 334 nm,the cloum temperature being 30 ℃.Results: The linear response range from 40.44~647.04 ng(r=0.999 8,n=5)of chlorogenic acid, 19.50~311.92 ng(r=0.999 7,n=5)of luteolin-7-O-β-D-glucoside, 253.62~4 057.82 ng (r=0.999 9,n=5)of Linarin. The average recoveries (n=6) chlorogenic acid,luteolin-7-O-β-D-glucoside,and Linarin were 100.96%,99.99%,and 98.94 % respectively. The average recoveries of chlorogenic acid,luteolin-7-O-β-D-glucoside,and Linarin were 100.96%,99.99%,and 98.94 % with RSD less than 2.0%.Conclusion: The method is simple ,accurate, reproducible and stable , and can be used for the quality control of Flos Chrysanthemi Indici. |
keywords:Chrysanthemi Indici Flos RP- HPLC Chlorogenic acid Luteolin-7-O-β-D-glucoside Linarin |
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